AI Article Synopsis
- Scanning electrochemical microscopy (SECM) was utilized to create and evaluate HRP microarrays on glass slides, enhancing our understanding of the enzyme's catalytic activity.
- By employing a combination of glutaraldehyde crosslinking and SECM for micro-deposition, researchers patterned lines of HRP on amino-modified slides.
- The study found that higher enzymatic activity resulted in significant positive feedback signals, while denatured HRP or non-coated slides showed negative feedback, and optimization of various conditions led to a detection limit of 1.2 × 10(-12) mol ml(-1) for benzoquinone.
Article Abstract
Scanning electrochemical microscopy (SECM) has been used to image and study the catalytic activity of horseradish peroxidase (HRP) immobilised in a patterned fashion onto glass slides. Microarrays of HRP islands could be deposited on amino-modified glass slides using glutaraldehyde crosslinking combined with the SECM being used as a micro-deposition device. The enzymatic activity of the immobilised enzyme on the surface was in the presence of its substrate observed to give rise to substantial positive feedback between the slide and the SECM microelectrode tip. Conversely when either blank slides - or slides coated with HRP which had been subsequently thermally denatured were utilised, these showed negative feedback effects. Various conditions such as enzyme concentration, incubation time and substrate concentration were systematically varied to optimise sensitivity. Regular arrays of HRP could be assembled and when imaged, displayed lower limits of detection of 1.2 × 10(-12) mol ml(-1) of benzoquinone.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/c1an15589j | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A novel sonogel-Leucine electrochemical sensor for the detection of neuroblastoma biomarker in human urine and synthetic cerebrospinal fluid.
Talanta
December 2024
Laboratory of Materials Engineering and Sustainable Energy (LMESE), Faculty of Science, Abdelmalek Essaadi University, B.P. 2121, 93002, Tetouan, Morocco. Electronic address:
A new Sonogel-Carbon (SNGC) electrode modified with an amino acid (l-leucine; Leu) has been developed for the sensitive detection of homovanillic acid (HVA). Electrochemical properties of leucine-modified Sonogel-Carbon (SNGC-Leu) were characterized using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Comprehensive characterization using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), Fourier-transform infrared spectroscopy (FTIR), and powder X-ray diffraction (XRD) was conducted to investigate the structure of both SNGC-Leu and SNGC electrodes.
View Article and Find Full Text PDFMorphology control and enhanced activity of (Cu-S)MOF@ZnS heterostructures for photocatalytic H production.
J Colloid Interface Sci
December 2024
Department of Chemistry, Fu Jen Catholic University, New Taipei City 24205, Taiwan.
A novel metal-organic framework (MOF), (Cu-S)MOF, with a copper-sulfur planar structure was applied to photocatalytic H production application. (Cu-S)MOF@ZnS nanocomposite was synthesized using a microwave-assisted hydrothermal approach. The formation of (Cu-S)MOF and wurtzite ZnS in the composite nanoparticles was analyzed by X-ray diffraction (XRD), field emission-scanning electron microscopy (FESEM), and high-resolution transmission electron microscope (HRTEM).
View Article and Find Full Text PDFElectrochemical polymerized DL-phenylalanine modified carbon nanotube sensor for the selective and sensitive determination of caffeic acid with riboflavin.
Sci Rep
December 2024
Department of Civil, Environmental and Mechanical Engineering, University of Trento, Via Mesiano, 77, 38123, Trento, Italy.
In this study, DL-phenylalanine modified with a multiwall carbon nanotube paste electrode is used as advanced electrochemical sensor for analysing of 0.1 mM caffeic acid (CFA) with simultaneous detection of riboflavin (RFN). The developed sensors include electrochemically polymerized DL-phenylalanine (DL-PA) modified multiwall carbon nanotube paste electrode [DL-PAMMCNTPE] and bare multiwall carbon nanotube paste electrode [BMCNTPE].
View Article and Find Full Text PDFElectrodeposition of Nanostructured Metals on n-Silicon and Insights into Rhodium Deposition.
Nanomaterials (Basel)
December 2024
Dipartimento di Chimica "Ugo Schiff", Università degli Studi di Firenze, Via della Lastruccia 3, 50019 Sesto Fiorentino, Italy.
In this study, we investigate the electrodeposition of various metals on silicon. Mn, Co, Ni, Ru, Pd, Rh, and Pt were identified as promising candidates for controlled electrodeposition onto silicon. Electrochemical evaluations employing cyclic voltammetry, Scanning Electron Microscopy (SEM) associated with energy-dispersive X-Ray Spectroscopy (SEM-EDS), and X-Ray Photoelectron Spectroscopy (XPS) techniques confirmed the deposition of Pd, Rh, and Pt as nanoparticles.
View Article and Find Full Text PDFOptimization for the Process Parameters of Nickel-Titanium Nitride Composites Fabricated via Jet Pulse Electrodeposition.
Nanomaterials (Basel)
December 2024
College of Mechanical Science and Engineering, Northeast Petroleum University, Daqing 163318, China.
The corrosion resistance of nickel-titanium nitride (Ni/TiN) composites is significantly influenced by the operation parameters during the jet pulse electrodeposition (JPE) process. The effect of current density, jet rate, TiN concentration, and duty cycle impact on the anti-corrosion property of Ni/TiN composites were investigated and optimized using the response surface method (RSM). After the optimization of the operation parameters, the corrosion current of Ni/TiN composites decreased from 9.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!