AI Article Synopsis
- * The study compared the effectiveness of the DiversiLab™ system (rep-PCR) with traditional IS6110-RFLP analysis to identify Mycobacterium tuberculosis in 89 clinical isolates from Korea.
- * The DiversiLab system successfully grouped the isolates into 8 categories, showing quick and reliable results, and is suggested for molecular epidemiological studies of tuberculosis in Korea.
Article Abstract
Tuberculosis remains a severe public health problem worldwide. Presently, genotyping is used for conducting epidemiologic and clinical studies on tuberculosis cases. We evaluated the efficacy of the repetitive sequence-based PCR (rep-PCR)-based DiversiLab™ system (bioMérieux, France) over the IS6110-restriction fragment length polymorphism analysis for detecting Mycobacterium tuberculosis. In all, 89 clinical M. tuberculosis isolates collected nationwide from Korea were used. The DiversiLab system allocated the 89 isolates to 8 groups with 1 unique isolate when a similarity level of 95% was applied. Seventy-six isolates of the Beijing family and 13 isolates of non-Beijing family strains were irregularly distributed regardless of rep-PCR groups. The DiversiLab system generated a rapid, sensitive, and standardized result. It can be used to conduct molecular epidemiologic studies to identify clinical M. tuberculosis isolates in Korea.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3190008 | PMC |
| http://dx.doi.org/10.3343/kjlm.2011.31.4.282 | DOI Listing |
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