We evaluated the performance and the cost of chromogenic medium Uriselect4 agar with regard to the standard medium for the detection and identification of urinary tract pathogens. A total of 503 clinical urine specimens containing leucocytes greater or equal to 104/mL were analysed prospectively, in parallel by two different persons on blood agar (GS) and Uriselect4 according to the manufacturers' instructions. Of the 503 urine specimens tested, 210 gave a positive culture on Uriselect4 versus 181 on GS. The majority of bacterial species grew on both media; enterobacteria grew on Uriselect4 better than GS. The identification of Escherichia coli (E. coli), Proteus mirabilis (P. mirabilis), KES group and Enterococcus faecalis (E. faecalis) did not require the use of galleries Api and has a gain of 24 h. Positive pure cultures on Uriselect4 corresponding to negative cultures of GS were noted in 17 ases. Conversely, in seven cases a positive pure culture on GS was noted while the corresponding Uriselect4 cultures were negative. The cost of identification on GS (including the cost of galleries Api), was about two times higher than Uriselect4. Uriselect4 medium isolates the most frequent urinary tract pathogens and identify them so almost immediately, with a lower cost.
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http://dx.doi.org/10.1684/abc.2011.0615 | DOI Listing |
Rev Esp Quimioter
February 2024
José Gutiérrez-Fernández. Laboratorio de Microbiología. Avenida de las Fuerzas Armadas, 2. E-18012. Granada, Spain.
Objective: Non-albicans Candida species, such as Candida kefyr, are emerging pathogens. Chromogenic media are highly useful for the diagnosis of urinary tract infections (UTIs). The aim was to describe the behavior of this specie on a non-specific chromogenic medium.
View Article and Find Full Text PDFIndian J Med Microbiol
March 2023
Higher School of Biological and Food Technology (ESTBA)/Laboratory of Microbiology and Quality Control of Foodstuffs (LAMICODA)/Research Unit in Immunology and Immunomodulation (UR2IM), University of Lome, Togo. Electronic address:
Purpose: The Cytobacteriological Examination of Urine (CBEU) is one of the most requested microbiological analyses in the medical bacteriology laboratory. According to the ISO 15189 standard, Medical Biology Laboratory (MBL) must validate the techniques before their use. It is in this perspective that, within the Medical Bacteriology Laboratory of the INH of Lome, we have carried out the on-site verification of the CBEU method using UriSelect4 chromogenic medium at the INH of Lomé.
View Article and Find Full Text PDFAnn Biol Clin (Paris)
February 2020
Laboratoire de biologie clinique, Institut National « Zouheir Kallel » de nutrition et de technologie alimentaire, Tunis, Tunisie, Faculté de pharmacie, Université de Monastir, Monastir, Tunisie.
Rapid and accurate identification of pathogens involved in urinary tract infections helps to guide antimicrobial therapy. Chromogenic agars provide presumptive identification directly from primary isolation media. They have been intended to make the bacterial isolation and identification process easier and faster.
View Article and Find Full Text PDFJ Clin Microbiol
August 2015
Laboratory of Infectious Agents and Hygiene, University Hospital of Saint-Etienne, Saint-Etienne, France GIMAP EA 3064 (Groupe Immunité des Muqueuses et Agents Pathogènes), Faculty of Medicine of Saint-Etienne, University of Lyon, Saint-Etienne, France.
Four chromogenic media were compared for their ability to detect urinary tract pathogens in 299 urine specimens, of which 175 were found positive, allowing the growth of 279 microorganisms. After 18 to 24 h of incubation, the CPS ID4, CPSE, CPSO (bioMérieux), and UriSelect4 (Bio-Rad) media showed sensitivities of 97.1%, 99.
View Article and Find Full Text PDFAnn Biol Clin (Paris)
January 2015
Plateau technique de microbiologie, Laboratoire de bactériologie, CHU Strasbourg, France.
Escherichia coli is the most common bacterial cause of urinary tract infections. Its rapid and specific identification in urine samples represents a major challenge within the rendering results and optimizing the management of the patient. We aimed to compare the sensitivity and specificity of two commercially available chromogenic media for E.
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