Objective: To develop an HPLC method for content determination of protocatechuic acid, chlorogenic acid and caffeic acid in Zhuang medicine Blumea megacephala (Randeria), and explore the content variation of the 3 components of the herbs harvesting in different months, and provide the scientific basis of reasonable application.
Method: The determination was carried out on a Shimadzu VP-ODS column (4.6 mm x 250 mm, 5 microm) eluted with acetonitrile and water containing 0.1% phosphoric acid in a linear gradient elution. The flow rate was 1 mL min(-1), and the detected wavelength was set at 258, 327nm.
Result: The peak areas and the concentrations of the three components had good linear relationship in the range of 1.7-17 mg x L(-1) for protocatechuic acid, 15.6-156 mg x L(-1) for chlorogenic acid, 3.96-39.6 mg x L(-1) for caffeic acid. The average recoveries were 103.4%, 102.2%, 98.5%, respectively.
Conclusion: The method was proved to be simple, accurate and used for the quality evaluation of Blumea megacephala (Randeria).
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Chemical profiling and rapid discrimination of and by UPLC-Q-Exactive-MS/MS and HPLC.
Chin Herb Med
April 2023
Key Laboratory of Qinghai-Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization of National Ethnic Affairs Commission, Chengdu 610225, China.
Objective: To rapidly identify the two morphologies and chemical properties of similar herbal medicines, and as the basis for chemical constituent analysis.
Methods: UPLC-Q-Exactive-MS/MS was utilized for profiling and identification of the constituents in and . Chemical pattern recognition (CPR) was further used to compare and distinguish the two herbs and to identify their potential characteristic markers.
[New records of medicinal plants in Hubei].
Zhongguo Zhong Yao Za Zhi
November 2017
College of Biology and Environmental Sciences, Jishou University, Jishou 416000, China.
In this paper, we make a report on new records of medicinal plants in Hubei, which include one newly recorded genera and seven newly recorded species and a newly recorded variety. The newly recorded genera is Anoectochilus and its corresponding species is Anoectochilus roxburghii; These newly recorded species are Euphorbia micractina, Astragalus wulingensis, Blumea megacephala, Potentilla saundersiana, Blumea formosana, Lycoris houdyshelii and Colocasia gigantea ; The newly recorded variety is Neottia puberula var. maculata.
View Article and Find Full Text PDF[Determination of three chemical constituents in zhuang medicine Blumea megacephala (Randeria) by HPLC].
Zhongguo Zhong Yao Za Zhi
June 2011
Guangxi College of Traditional Chinese Medicine, Nanning 530001, China.
Objective: To develop an HPLC method for content determination of protocatechuic acid, chlorogenic acid and caffeic acid in Zhuang medicine Blumea megacephala (Randeria), and explore the content variation of the 3 components of the herbs harvesting in different months, and provide the scientific basis of reasonable application.
Method: The determination was carried out on a Shimadzu VP-ODS column (4.6 mm x 250 mm, 5 microm) eluted with acetonitrile and water containing 0.
Chemical composition and antimicrobial activities of essential oil of Blumea megacephala.
EXCLI J
May 2011
College of Food and Bioengineering, South China University of Technology, Wushan Road 381, Guangzhou 510641, China.
essential oil, obtained through steam distillation of samples collected from the Shiwang Mountains in Guangxi Province, China, was analyzed using GC-FID and GC-MS. Among the 65 compounds identified in the oil, the main compounds were borneol (13.6 %), β-caryophyllene (9.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!