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A novel method for expressing and purifying large quantities of functional and stable human voltage-gated proton channel (hH1).
Protein Sci
February 2025
Cell Physiology and Molecular Biophysics Department, Center for Membrane Protein Research, Texas Tech University Health Sciences Center, Lubbock, Texas, USA.
Purifying membrane proteins has been the limiting step for studying their structure and function. The challenges of the process include the low expression levels in heterologous systems and the requirement for their biochemical stabilization in solution. The human voltage-gated proton channel (hH1) is a good example of that: the published protocols to express and purify hH1 produce low protein quantities at high costs, which is an issue for systematically characterizing its structure and function.
View Article and Find Full Text PDFExpression and characterization of the complete cyanophage genome PP in the heterologous host Synechococcus elongatus PCC 7942.
Int J Biol Macromol
January 2025
School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, PR China; Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, PR China; Frontier Science Center for Synthetic Biology (Ministry of Education), Tianjin University, Tianjin 300072, PR China. Electronic address:
In this study, we successfully integrated the full-length genome of the cyanophage PP into the non-host cyanobacterium Synechococcus elongatus PCC 7942, facilitated by conjugation via Escherichia coli. To address the challenge posed by the toxic open reading frames (ORFs) of PP in E. coli, we first identified and characterized three toxic ORFs.
View Article and Find Full Text PDFHeterologous expression of a recombinant ACE inhibitory peptide LYPVK and its potential antihypertensive action mechanism.
Int J Biol Macromol
January 2025
College of Food Science and Engineering, Ocean University of China, Qingdao 266404, China. Electronic address:
Enzymatic hydrolysis approach is commonly employed for preparation of active peptides, while the limited purity and yield of produced peptides hinder further development of action mechanisms. This study presents the biotechnological approach for the efficient production of recombinant angiotensin converting enzyme (ACE) inhibitory peptide LYPVK and investigates its potential antihypertensive action mechanism. DNA encoding sequence of recombinant peptide was designed to form in tandem, which was expressed in Escherichia coli BL21 (DE3).
View Article and Find Full Text PDFGenomic, Evolutionary, and Pathogenic Characterization of a New in Traditional Chinese Medicine .
Viruses
January 2025
Research Center for Life Sciences Computing, Zhejiang Lab, Hangzhou 311100, China.
, a medicinal herbaceous plant documented in the Chinese Pharmacopoeia, is a promising candidate for research into plant-derived pharmaceuticals. However, the study of newly emerging viruses that threaten the cultivation of remains limited. In this study, plants exhibiting symptoms such as leaf yellowing, mottled leaves, and vein chlorosis were collected and subjected to RNA sequencing to identify potential viral pathogens.
View Article and Find Full Text PDFThe Functional Identification of the Gene in the Kidney of .
Int J Mol Sci
January 2025
Xinjiang Production and Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, College of Life Science, Tarim University, Alar 843300, China.
This study aims to identify the function of the () gene in the kidneys of . CYP2E1 is a significant metabolic enzyme involved in the metabolism of various endogenous and exogenous compounds and is associated with the occurrence and progression of multiple diseases. Given 's ability to survive in the extremely arid , we hypothesize that CYP2E1 in its kidneys plays a crucial role in adaptability.
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