Glucosinolates (GLs) present in root, seed, and leaf extracts of Pentadiplandra brazzeana Baillon were characterized and quantified according to the ISO 9167-1 method based on the HPLC analysis of desulfo-GLs. The analyses were complemented by GC-MS analyses of the isothiocyanates (ITCs) generated from GL degradation by myrosinase. Glucotropaeolin (1a), glucolimnanthin (2a), and glucoaubrietin (3a) were shown to be present in the root extract, whereas the seed mainly contained 3a. 3,4-Dimethoxybenzyl GL (4a), glucobrassicin (5a) and traces of 1a were detected in the leaf extract. The products were fully characterized as their desulfo-counterparts by spectroscopic techniques.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.phytochem.2011.09.006 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
[Analysis of the properties of Komagataella phaffii CF-st401, a genetically modified producer of the sweet protein brazzein by using in silico methods].
Vopr Pitan
October 2024
Federal Research Centre of Nutrition, Biotechnology and Food Safety, 109240, Moscow, Russian Federation.
Article Synopsis
- Sweeteners are commonly used to reduce sugar intake in diets, but they often don't taste as good as natural sugars, leading to the creation of a new sweet protein called brazzein, produced from the strain Komagataella phaffii CF-st401.* -
- The research focused on assessing the safety of this genetically modified microorganism (K. phaffii CF-st401) using in silico methods, analyzing its plasmid and DNA structure for potential risks like toxin production, antibiotic resistance, and allergenicity.* -
- Findings showed that over 70% of the genetic material responsible for producing brazzein in K. phaffii CF-st401 is similar to the reference protein from the plant Pent
Novel gnd_v2 Fusion Tag and Engineered TEV Protease Enable Efficient Production of Brazzein.
J Microbiol Biotechnol
November 2024
CCZU-JITRI joint Bio-X Lab, School of Pharmacy & School of Biological and Food Engineering, Changzhou University, 213164, Changzhou, Jiangsu Province, P.R. China.
Protein solubility and purification challenges often hinder the large-scale production of valuable proteins like brazzein, a potent sweet protein with significant health benefits and commercial potential. This study introduces two novel tools to overcome protein expression and purification bottlenecks: a gnd_v2 fusion tag and an engineered Tobacco Etch Virus (TEV) protease. The gnd_v2 tag, derived from 6-phosphogluconate dehydrogenase, was engineered to improve the soluble expression of brazzein.
View Article and Find Full Text PDFDetermination of Hydrogen Bonds in GROMACS: A New Implementation to Overcome Memory Limitation.
J Chem Inf Model
August 2024
Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre "Kurchatov Institute", Gatchina 188300, Russia.
This work introduces a new faster implementation of the hydrogen bond network (complex arrangement of hydrogen bonds between or within molecules) search algorithm in biomacromolecules and their environment. Existing implementation of such an algorithm in GROMACS [Abraham et al. .
View Article and Find Full Text PDFRecombinant expression and tryptophan-assisted analysis of human sweet taste receptor T1R3's extracellular domain in sweetener interaction studies.
Prep Biochem Biotechnol
October 2024
Department of Chemistry, College of Natural Sciences, Chung-Ang University, Seoul, Korea.
The human palate can discern multiple tastes; however, it predominantly perceives five fundamental flavors: sweetness, saltiness, sourness, bitterness, and umami. Sweetness is primarily mediated through the sweet taste receptor, a membrane-bound heterodimeric structure comprising T1R2-T1R3. However, unraveling the structural and mechanistic intricacies of the sweet taste receptor has proven challenging.
View Article and Find Full Text PDFIn vitro anti-yeast activity, kinetics and mechanism of action of essential oils from two cameroonian medicinal plants.
BMC Complement Med Ther
April 2023
Department of Microbiology, Laboratory of Microbiology, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon.
Background: Treatment of Candida infections have become increasingly difficult due to antifungal drug resistance, which has drawn attention toward the search for innovative and effective drugs. This study aimed to assess the activity of essential oils (EOs) from Pentadiplandra brazzeana Baillon (PB) root and Drypetes gossweileri S. Moore (DG) stem bark against Candida albicans and Candida parapsilopsis strains, and determine their antifungal mechanism when tested alone or combined.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!