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http://dx.doi.org/10.1042/bst0180332 | DOI Listing |
Assessments of genetic diversity, structure, history, and effective population size ( ) are critical for the conservation of imperiled populations. The lesser prairie-chicken () has experienced declines due to habitat loss, degradation, and fragmentation in addition to substantial population fluctuations with unknown effects on genetic diversity. Our objectives were to: (i) compare genetic diversity across three temporally discrete sampling periods (2002, 2007-2010, and 2013-2014) that are characterized by low or high population abundance; (ii) examine genetic diversity at lek and lek cluster spatial scales; (ii) identify potential bottlenecks and characterize genetic structure and relatedness; and (iii) estimate the regional .
View Article and Find Full Text PDFAnn Bot
January 2025
Unit of Ecological Genetics, Institute of Forest Biodiversity and Nature Conservation, Austrian Research Centre for Forests (BFW), Seckendorff-Gudent-Weg 8, Vienna, Vienna.
Background And Aims: Torminalis glaberrima (Gand.) Sennikov & Kurtto is a European tree species currently underutilized in forestry, valued for its high-quality wood and contribution to ecosystem stability. Despite a projected range expansion as climate change progresses, current population fragmentation levels may inhibit the species' ability to migrate and stabilize fragile forest ecosystems.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
January 2025
Department of Chemical Engineering, University of Florida, Gainesville, FL 32611.
We describe a microfluidic device to extract DNA from a cell lysate, without the need for centrifuges, magnetic beads, or gels. Instead, separation is driven by transverse migration of DNA, which occurs when a polyelectrolyte solution flowing through a microfluidic channel is subjected to an electric field. The coupling of the weak shearing with the axial electric field is highly selective for long, flexible, charged molecules, of which DNA is the sole example in a typical cell lysate.
View Article and Find Full Text PDFJ Agric Food Chem
January 2025
Engineering Research Center of Protection and Utilization of Plant Resources, College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, Liaoning Province 110866, China.
1-Aminocyclopropane-1-carboxylic acid (ACC) is a direct precursor of phytohormone ethylene. We used a phenyl isothiocyanate (PITC) derivatization modification method combined with spectrographic analysis to isolate and identify three products of the derivatization reactions of ACC and PITC. The MRM mode of UPLC-MS/MS was used to establish the analysis of 6-phenyl-5-thioxo-4,6-diazaspiro[2.
View Article and Find Full Text PDFFront Reprod Health
January 2025
Assisted Reproductive Technology Center, Maternity and Reproductive Health Hospital les Orangers of IbnSina University Hospital, Rabat, Morocco.
Introduction: Infertility has significant implications on health. It affects physical, emotional, and social wellbeing. In Morocco, about 12% of couples or live with infertility.
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