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In vivo cell biology using Gal4-mediated multicolor subcellular labeling in zebrafish. | LitMetric

AI Article Synopsis

  • The behavior of cells is influenced by their internal components, and visualizing multiple organelles in living organisms can deepen our understanding of development.
  • Researchers created a Gal4-based system to label and track various cell types in transparent zebrafish embryos, allowing for real-time observation using advanced microscopy.
  • Recent findings indicate that the centrosome does not always direct neuron movement or axon formation in zebrafish, and new data on centrosomal and microtubule dynamics during early neuroepithelial cell divisions is presented.

Article Abstract

The behavior of a cell is determined by the interplay of its subcellular components. Thus, being able to simultaneously visualize several organelles inside cells within the natural context of a living organism could greatly enhance our understanding of developmental processes. We have established a Gal4-based system for the simultaneous and cell type specific expression of multiple subcellular labels in transparent zebrafish embryos. This system offers the opportunity to follow intracellular developmental processes in a live vertebrate organism using confocal fluorescence time-lapse microscopy. Using this approach we recently showed that the centrosome neither persistently leads migration nor determines the site of axonogenesis in migrating neurons in the zebrafish cerebellum in vivo. Here we present additional in vivo findings about the centrosomal and microtubule dynamics of neuroepithelial cells during mitotic cleavages at early neural tube stages.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3187902PMC
http://dx.doi.org/10.4151/cib.4.3.15037DOI Listing

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