AI Article Synopsis
- - The study analyzed the genetic evolution of the VP1 protein in enterovirus type 71 (EV71) strains from Shenzhen, focusing on their nucleotide sequences and phylogenetic relationships.
- - A total of 35 strains showed a VP1 sequence homogeneity of 92.1%-100%, with significant variations when compared to subgenotypes A and B, indicating a shift in prevalent strains from cluster C4b to C4a over the years.
- - Yearly analysis revealed a gradual increase in sequence homogeneity from 2003 to 2008, alongside a specific mutation in the VP1 nucleotide at position 66 leading to an amino acid change, suggesting potential evolutionary adaptations of EV71.
Article Abstract
Objective: Genetic evolution of VP1 of enterovirus type 71 in Shenzhen were analyzed.
Methods: All samples were tested by RT-PCR using EV71 specific primer. The VP1 of EV71 were amplified and sequenced. A phylogenetic tree was constructed by comparison of the sequences with subgenotype A, B and C using DNAStar, BioEdit and Mega 3.1 software.
Results: Among 35 strains, the homogeneity of the VP1 nucleotide sequence was between 92.1%-100%. The homogeneity of the VP1 nucleotide sequence with subgenotype A and B was between 81.4% -91.1%. The VP1 nucleotide sequence of 35 strains of Shenzhen shared between 93% -97.4% identity with cluster C4. The prevalence strains of EV71 were cluster C4b from 1998 to 2004, and gradually moved to C4a since 2003. All of EV71 were C4b from 2006 to 2008. Also, the homogeneity of the VP1 nucleotide sequence with Anhui FY23 EV71 strain were 94.5% -94.7%, 95.7% -95.8%, 96.2%, 95.4% -97.5%, 96.3% -99.2% from 2003 to 2008. It shows that the homogeneity was increased year by year. There was a mutation (A --> C) at No. 66 nucleotide of VP1 of EV71 that two strains were isolated in 2003 and 8 strains in 2008, that caused amino acid mutation (Q --> H) at No. 22 of VP1.
Conclusion: EV71 C4b was gradually moved to C4a from 1998 to 2008. There was a missense mutation at No. 66 nucleotide of VP1.
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