The effect on endoreduplication in sugar beet (Beta vulgaris L.) seedlings of five plant hormones in MS medium, ethylene, 24-epibrassinolide (EBR), gibberellic acid (GA(3) ), kinetin and 1-naphthaleneacetic acid (NAA), as well as a combination of kinetin and NAA at two different concentrations, was studied using flow cytometry. Analyses of DNA content in nuclei of the root, hypocotyl and cotyledons of seedlings growing in vitro were performed during their early development, starting from when the root was 0.5-1.0 cm long until expansion of the first pair of leaves. The proportions of nuclei with different DNA contents were established and the mean C-value calculated. The presence of exogenous plant hormones changed endoreduplication intensity, although to different extents, depending on the organ and developmental stage. Ethylene and NAA stimulated the process, while EBR and kinetin suppressed it and GA did not clearly affect it.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1438-8677.2011.00477.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The midgut epithelium of mosquitoes adjusts cell proliferation and endoreplication to respond to physiological challenges.
BMC Biol
January 2024
Department of Entomology, College of Agriculture and Life Sciences, Cornell Institute of Host-Microbe Interactions and Disease, Cornell University, Ithaca, NY, 14852, USA.
Background: Hematophagous mosquitoes transmit many pathogens that cause human diseases. Pathogen acquisition and transmission occur when female mosquitoes blood feed to acquire nutrients for reproduction. The midgut epithelium of mosquitoes serves as the point of entry for transmissible viruses and parasites.
View Article and Find Full Text PDFTranscriptomic analysis of Rhizobium leguminosarum bacteroids in determinate and indeterminate nodules.
Microb Genom
February 2019
1John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
Two common classes of nitrogen-fixing legume root nodules are those that have determinate or indeterminate meristems, as in Phaseolus bean and pea, respectively. In indeterminate nodules, rhizobia terminally differentiate into bacteroids with endoreduplicated genomes, whereas bacteroids from determinate nodules are less differentiated and can regrow. We used RNA sequencing to compare bacteroid gene expression in determinate and indeterminate nodules using two Rhizobium leguminosarum strains whose genomes differ due to replacement of the symbiosis (Sym) plasmid pRP2 (strain Rlp4292) with pRL1 (strain RlvA34), thereby switching symbiosis hosts from Phaseolus bean (determinate nodules) to pea (indeterminate nodules).
View Article and Find Full Text PDFA Transcript and Metabolite Atlas of Blackcurrant Fruit Development Highlights Hormonal Regulation and Reveals the Role of Key Transcription Factors.
Front Plant Sci
August 2018
Cell and Molecular Sciences, The James Hutton Institute, Dundee, United Kingdom.
Blackcurrant fruit collected at six stages of development were assessed for changes in gene expression using custom whole transcriptome microarrays and for variation in metabolite content using a combination of liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry. Principal components analysis demonstrated that fruit development could be clearly defined according to their transcript or metabolite profiles. During early developmental stages, metabolite profiles were dominated by amino acids and tannins, whilst transcript profiles were enriched in functions associated with cell division, anatomical structure morphogenesis and cell wall metabolism.
View Article and Find Full Text PDFPEG Induces High Expression of the Cell Cycle Checkpoint Gene in Embryogenic Callus of : Potential Link between Cell Cycle Checkpoint Regulation and Osmotic Stress.
Front Plant Sci
September 2017
Agroécologie, AgroSup Dijon, Institut National de la Recherche Agronomique (INRA), University of Bourgogne Franche-ComtéDijon, France.
Polyethylene glycol (PEG) can be used to mimic osmotic stress in plant tissue cultures to study mechanisms of tolerance. The aim of this experiment was to investigate the effects of PEG (M.W.
View Article and Find Full Text PDFLeaf development: a cellular perspective.
Front Plant Sci
August 2014
Laboratory for Molecular Plant Physiology and Biotechnology, Department of Biology, University of Antwerp Antwerp, Belgium.
Through its photosynthetic capacity the leaf provides the basis for growth of the whole plant. In order to improve crops for higher productivity and resistance for future climate scenarios, it is important to obtain a mechanistic understanding of leaf growth and development and the effect of genetic and environmental factors on the process. Cells are both the basic building blocks of the leaf and the regulatory units that integrate genetic and environmental information into the developmental program.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!