AI Article Synopsis
- An ethanol extract from Pyrola rotundifolia stimulates AMPK phosphorylation in C2C12 muscle cells.
- Bioassay-guided fractionation isolated a compound, 2-methyl-7-hydroxymethyl-1,4-naphthoquinone, which exhibited dose-dependent AMPK activation, particularly at concentrations between 2.5-20 μg/ml.
- At 10 μg/ml, this compound increased AMPK phosphorylation by about four times, showing similar effectiveness to other natural AMPK activators.
Article Abstract
An aqueous ethanol extract of Pyrola rotundifolia L. induced AMP-activated protein kinase (AMPK) phosphorylation in C2C12 myotubes. The bioassay-guided fractionation of the extract led to the isolation a 2-methyl-7-hydroxymethyl-1,4-naphthoquinone, or a 7'-hydroxy-chimaphilin, which showed concentration-dependent AMPK phosphorylation activity at 2.5-20 μg/ml. At a concentration of 10 μg/ml (50 μM), an approximately four-fold increase in the AMPKα(Thr¹⁷²) phosphorylation level was observed. The stimulatory effect of naphthoquinone on AMPK activity was comparable to that of known compounds found in natural sources that activate the AMPK signaling pathway.
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| http://dx.doi.org/10.1016/j.fitote.2011.09.005 | DOI Listing |
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