An intracellular approach for monitoring protein production in Staphylococcus aureus is described. mCherry, fused to the dodecapeptide Tip, was capable of inducing tetracycline repressor (TetR). Time- and concentration-dependent production of mCherry could be correlated to TetR-controlled GFPmut2 activity. This approach can potentially be extended to native S. aureus proteins.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815279 | PMC |
| http://dx.doi.org/10.1111/j.1751-7915.2011.00304.x | DOI Listing |
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