AI Article Synopsis
- The study analyzed the electroanalytical performance of different types of chemically-modified graphenes (CMGs) with varying defect densities and oxygen groups in detecting the oxidation of DNA bases.
- Both adenine and cytosine showed improved sensitivity across all CMGs, while guanine and thymine were more effectively detected using electrochemically reduced and thermally reduced graphene oxides compared to conventional glassy carbon electrodes.
- The research highlighted how variations in surface structures and functionalities of CMGs significantly impact their effectiveness in DNA base detection, paving the way for future electrochemical devices for DNA analysis.
Article Abstract
We studied the electroanalytical performances of chemically-modified graphenes (CMGs) containing different defect densities and amounts of oxygen-containing groups, namely graphite oxide (GPO), graphene oxide (GO), thermally reduced graphene oxide (TR-GO) and electrochemically reduced graphene oxide (ER-GO) by comparing the sensitivity, selectivity, linearity and repeatability towards the oxidation of DNA bases. We have observed that for differential pulse voltammetric (DPV) detection of adenine and cytosine, all CMGs showed enhanced sensitivity to oxidation, while for guanine and thymine, ER-GO and TR-GO exhibited much improved sensitivity over bare glassy carbon (GC) as well as over GPO and GO. There is also significant selectivity enhancement when using GPO for adenine and TR-GO for thymine. Our results have uncovered that the differences in surface functionalities, structure and defects of various CMGs largely influence their electrochemical behaviour in detecting the oxidation of DNA bases. The findings in this report will provide a useful guide for the future development of label-free electrochemical devices for DNA analysis.
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| http://dx.doi.org/10.1039/c1an15631d | DOI Listing |
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