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Dietary profiles of wild carnivores and Blastocystis occurrence: The case of the endangered Iberian lynx (Lynx pardinus) and systematic review.
Res Vet Sci
December 2024
CIBERINFEC, ISCIII - CIBER Infectious Diseases, Health Institute Carlos III, Madrid, Spain; Parasitology Reference and Research Laboratory, Spanish National Centre for Microbiology, Health Institute Carlos III, Majadahonda, Madrid, Spain.
Recent molecular and metagenomic studies have revealed that the obligate anaerobic protist Blastocystis is found more prevalently and with higher subtype diversities in herbivore species than in carnivore species. However, information on wild carnivore species is scarce. Here, we investigated the presence of Blastocystis by molecular methods in fecal DNA samples of free-ranging and captive Iberian lynxes from Spain (n = 243) and Portugal (n = 30).
View Article and Find Full Text PDFCRISPR-Cas12a-driven aptasensor for sensitive detection of alternariol by using a personal glucose meter.
Talanta
January 2025
Institute of Quality Standard and Testing Technology of BAAFS, Beijing 100097, China. Electronic address:
Alternariol (AOH) has attracted much attention as an emerging toxin in edible herbs that can pose potential carcinogenic risks to human. However, the rapid detection of AOH to ensure food safety remains a challenge. Here, a CRISPR-Cas12a-mediated aptamer-based sensor (aptasensor) was proposed for the sensitive quantification of AOH by using a personal glucose meter.
View Article and Find Full Text PDFA matched case-control study of porcine group A and C rotaviruses in a swine farrowing production system.
Vet Microbiol
December 2024
Saint-Hyacinthe Research and Development Centre, Agriculture and Agri-Food Canada, 3600 Casavant Blvd. West, Saint-Hyacinthe, Québec J2S 8E3, Canada; Swine and Poultry Infectious Diseases Research Centre (CRIPA-FRQNT), Université de Montréal, 3200 Sicotte, Saint-Hyacinthe, Québec J2S 2M2, Canada. Electronic address:
Group A rotaviruses (RVA) and group C rotaviruses (RVC) are important enteric pathogens in swine. Comprehensive studies investigating porcine rotaviruses in Canada are necessary to enhance understanding of the frequency, impacts, and dynamics of these infections in swine herds. This study aims to estimate the prevalence of RVA and RVC, describe circulating strains, and assess the association of rotaviruses with diarrhea at the piglet, litter, and batch levels in Canadian farrowing swine productions.
View Article and Find Full Text PDFDNAzyme-mediated isothermal catalytic hairpin assembly for rapid and enzyme-free amplified detection of lead(Ⅱ) ion.
J Hazard Mater
January 2025
Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai Ocean University, Shanghai 201306, China; Laboratory of Quality and Safety Risk Assessment for Aquatic Product on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China; Shanghai Ocean University, Shanghai 201306, China. Electronic address:
The detection of heavy metal ions, particularly lead (Pb²⁺), in environmental samples is crucial for public health and safety. Current nucleic acid signal amplification methods for Pb²⁺ detection often rely on biological enzymes and are limited in applicability due to high costs, prolonged detection times, and nonspecific adsorption. In this study, we introduce an enzyme-free, DNAzyme-mediated isothermal catalytic hairpin assembly (DMICHA) assay, which combines a DNAzyme-based Pb²⁺ recognition module with a signal amplification process utilizing isothermal catalytic hairpin assembly (CHA).
View Article and Find Full Text PDFUnveiling soil-borne antibiotic resistome and their associated risks: A comparative study of antibiotic and non-antibiotic pharmaceutical factories.
J Hazard Mater
January 2025
Key Laboratory of Recycling and Eco-Treatment of Waste Biomass of Zhejiang Province, School of Environmental and Natural Resource, Zhejiang University of Science and Technology, Hangzhou, Zhejiang 310023, China.
Antibiotic resistance genes (ARGs) and antibiotic-resistant bacteria (ARB) are extensively documented within antibiotic pharmaceutical factories. Notably, non-antibiotic pharmaceuticals also represent a significant portion of the pharmaceuticals market. However, the comparative analyses of soil-borne ARG profiles and associated risks in different categories of pharmaceutical factories remain limited.
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