We report the concentration and purification of bacterial 16S rRNA by the use of a biotinylated DNA target-specific capture (TSC) probe. For both cultivated bacterial and urine specimens from urinary tract infection patients, TSC resulted in a 5- to 8-fold improvement in the sensitivity of bacterial detection in a 16S rRNA electrochemical sensor assay.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3232961 | PMC |
| http://dx.doi.org/10.1128/JCM.01261-11 | DOI Listing |
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