Aim: To analyze the microbiota shift in the distal esophagus of Sprague-Dawley rats fed a high-fat diet.
Methods: Twenty Sprague-Dawley rats were divided into high-fat diet and normal control groups of 10 rats each. The composition of microbiota in the mucosa from the distal esophagus was analyzed based on selective culture. A variety of Lactobacillus species were identified by molecular biological techniques. Bacterial DNA from Lactobacillus colonies was extracted, and 16S rDNA was amplified by PCR using bacterial universal primers. The amplified 16S rDNA products were separated by denaturing gradient gel electrophoresis (DGGE). Every single band was purified from the gel and sent to be sequenced.
Results: Based on mucosal bacterial culturing in the distal esophagus, Staphylococcus aureus was absent, and total anaerobes and Lactobacillus species were decreased significantly in the high-fat diet group compared with the normal control group (P < 0.01). Detailed DGGE analysis on the composition of Lactobacillus species in the distal esophagus revealed that Lactobacillus crispatus, Lactobacillus gasseri (L. gasseri) and Lactobacillus reuteri (L. reuteri) comprised the Lactobacillus species in the high-fat diet group, while the composition of Lactobacillus species in the normal control group consisted of L. gasseri, Lactobacillus jensenii and L. reuteri.
Conclusion: High-fat diet led to a mucosal microflora shift in the distal esophagus in rats, especially the composition of Lactobacillus species.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3158416 | PMC |
| http://dx.doi.org/10.3748/wjg.v17.i26.3151 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The sow vaginal and gut microbiota associated with longevity and reproductive performance.
J Anim Sci Biotechnol
January 2025
Department of Animal Science, University of Arkansas, Fayetteville, AR, USA.
Background: Sow longevity and reproductivity are essential in the modern swine industry. Although many studies have focused on the genetic and genomic factors for selection, little is known about the associations between the microbiome and sows with longevity in reproduction.
Results: In this study, we collected and sequenced rectal and vaginal swabs from 48 sows, nine of which completed up to four parities (U4P group), exhibiting reproductive longevity.
Microbiota of the Mammary Gland in Wistar Rats with Chemically Induced Breast Cancer after Treatment.
Bull Exp Biol Med
January 2025
Institute of Veterinary Medicine and Biotechnology, Novosibirsk State Agrarian University, Novosibirsk, Russia.
We conducted a comparative study of the mammary gland microbiota in female Wistar rats and the microbiota associated with breast cancer (BC) induced by the administration of N-methyl-N-nitrosourea, after surgical treatment, photodynamic therapy (PDT), and chemotherapy (CT). Selective nutrient media and a smear-fingerprint technique were used to study the microbiota. Staphylococcus, Streptococcus, and Lactobacillus were found in the mammary glands of intact rats.
View Article and Find Full Text PDFMaternal milk fat globule membrane enriched gut and circulating SCFAs to improve placental efficiency and fetal development in intrauterine growth restricted mice model.
Gut Microbes
December 2025
Department of Obstetrics and Gynecology and Reproductive Medicine, Peking University First Hospital, Beijing, China.
Intrauterine growth restriction (IUGR) caused by placental dysfunctions leads to fetal growth defects. Maternal microbiome and its metabolites have been reported to promote placental development. Milk fat globule membrane (MFGM) is known for its diverse bioactive functions, while the effects of gestational MFGM supplementation on the maternal gut microbiota, placental efficiency, and fetal development remained unclear.
View Article and Find Full Text PDFGut microbiota: A new key of understanding for Panax notoginseng against multiple disorders and biotransformation.
J Ethnopharmacol
January 2025
Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China. Electronic address:
Ethnopharmacological Relevance: Panax notoginseng (Burkill) F.H.Chen(P.
View Article and Find Full Text PDFThe Effects of Compound Starter Culture and Sugar and Soy Milk on the Quality and Probiotic Activity of Milk-Soy Mixed Yogurt.
J AOAC Int
January 2025
College of Food Science and Engineering, Jilin University, Changchun, 130062, China.
Background: Yogurt has emerged as an essential nutritional food in contemporary diets, and the development of new multi-component yogurt formulations has become a focal point of current research.
Objective: In this study, the effects of fermentation compounds and the addition of sugar, soy milk on the quality and probiotic activity of milk-soy mixed yogurt were studied to determine the optimal formation of mixed yogurt.
Methods: The various fermentation compounds (YO-MIX 883, Lactobacillus Casei complex starter cultures, Lactobacillus Paracasei compound starter cultures), different concentrations of milk-soy additions (0, 25, 50, 75, 100%) and sugar (2, 4, 6, 8%) were tested within each experimental group, and the pH, appropriate acidity, and total viable bacterial count of the fermented milk-soy mixed yogurt were determined throughout the fermentation and refrigeration processes.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!