Purpose: Bacterial community in dental plaque of elder people was analyzed to learn about the microhabitat composition and diversity.
Methods: Dental plaque samples were collected from 25 elders. PCR-based denaturing gradient gel electrophoresis (PCR-DGGE) was used to evaluate the microbial diversity by displaying PCR-generated 16SrDNA fragments that migrate at different distances, reflecting the different sequence of fragment. SPSS12.0 software was used to analyze the variance of genotypes between different groups of bacteria.
Results: Genotypes of bacteria in dental plaques in the root caries group was significantly more than the other two groups. Crown caries group and caries-free group had no significant difference.
Conclusions: The genetic diversity of the dental plaque microflora in the root caries group is significantly higher than coronal caries group and caries-free group.
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