The ability to apply precise inputs to signaling species in live cells would be transformative for interrogating and understanding complex cell-signaling systems. Here we report an 'optogenetic' method for applying custom signaling inputs using feedback control of a light-gated protein-protein interaction. We applied this strategy to perturb protein localization and phosphoinositide 3-kinase activity, generating time-varying signals and clamping signals to buffer against cell-to-cell variability or changes in pathway activity.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184382 | PMC |
| http://dx.doi.org/10.1038/nmeth.1700 | DOI Listing |
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