Aim: To synthesize and identify the hapten and complete antigens for Zearalenone for the purpose of preparation of anti-Zearalenone antibodies.
Methods: Zearalenone and carboxymethoxylamine hemihydrochloride were incubated to synthesize the hapten ZEN-oxime. The ZEN-oxime was identified by thin layer chromatography (TLC), high performance liquid chromatographic (HPLC) and liquid chromatography-tandem mass spectrometry (LC-MSn). The ZEN-oxime was further conjugated to bovine serum albumin (BSA) or ovalbumin (OVA) to form the complete antigens by NHS ester method. The complete antigens were identified by ultraviolet spectrum, 2, 4, 6-trinitrobenzene sulfonicacid method (TNBS) and immunological analysis.
Results: The results indicated that the hapten and complete antigens were prepared successfully.
Conclusion: The hapten and complete antigens for Zearalenone have been successfully synthesized and identified by several methods, which enables further preparation of anti-Zearalenone antibodies.
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