AI Article Synopsis
- - This study investigates how angiotensin-(1-7) (Ang-(1-7)) affects the transformation of rat kidney cells (epithelial-myofibroblast transdifferentiation) that is induced by angiotensin II (Ang II), along with how it influences the production of extracellular matrix components.
- - Methods included treating cultured rat kidney cells with Ang-(1-7) and Ang II over various time periods, then analyzing changes in protein and mRNA levels for markers like E-cadherin and α-SMA, as well as measuring collagen content using ELISA and real-time PCR.
- - Results showed that while Ang II lowered E-cadherin levels (linked to healthy cell
Article Abstract
Aim: To explore the influence of angiotensin-(1-7)[Ang-(1-7)] on angiotension II(Ang II) induced rat's tubular epithelial-myofibroblast transdifferentiation and the secretion of extracellular matrix.
Methods: The NRK52E were maintained and sub-cultured treated with Ang-(1-7) (10(-6); mmol/L) and Ang II(10(-6); mmol/L) for 24, 48, 72, 96 hours, we detect the protein expressions of E-cadherin and α-SMA by immunocytochemistry method; The content of Col I and FN in the cultured supernatant were measured by ELISA; The mRNA expression of E-cadherin, α-SMA, Col I and FN was detected by real-time PCR.
Results: Treat with ang II 96 h, the protein and mRNA expression of E-cadherin decreased significantly (P<0.05), but the protein and mRNA expression α-SMA, col I and FN increased significantly (P<0.05); treat with Ang II and Ang-(1-7), the protein and mRNA expression of E-cadherin increased significantly (P<0.05), but the protein and mRNA expression α-SMA, col I and FN decreased significantly (P<0.05).
Conclusion: Ang-(1-7) can inhibits Ang II-induced rat's tubular epithelial myofibroblast transdifferentiation and decrease the secretion of FN and Col I.
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