AI Article Synopsis
- Researchers exposed single-stranded DNA from the M13 mp10 phage to tritiated water to analyze the effects of low-dose-rate beta-irradiation on transfection in E. coli K12 JM101.
- The study found that as the radiation dose rate decreased, the transfection activity of the DNA also decreased significantly.
- Additionally, other oxidative species were identified in tritiated water, suggesting that lower dose rates may enhance DNA modification compared to reactive oxygen species production during competitive reactions.
Article Abstract
In order to study the low-dose-rate effect of tritium beta-irradiation, we exposed single-stranded DNA of M13 mp10 phage to purified tritiated water. The beta-irradiated DNA was then transfected to E. coli K12 JM101. The lower the radiation dose rate, the more the transfection activity decreased. After the low-dose-rate effect was confirmed, the characteristics of tritiated water were also studied. The formation of an active oxidative species different from .OH, HO2., O2-., and H2O2 was found in the tritiated water by using UV spectrometry and luminescence measurement. Attributing it to nescent oxygen by reason of the production of the epoxide from mesityl oxide, we discussed the low-dose-rate effect from the viewpoint that the yield of the DNA modification could be relatively higher than that of the O2 formation in a competition reaction when the dose rate was lower.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Skin absorption of metals derived from hydrogenated stainless particles in human skin: Results from the TITANS project.
Environ Pollut
January 2025
Department of Chemistry and Pharmaceutical Sciences, University of Trieste, Trieste, Italy.
Workers involved in the decommissioning and removal of radioactive material from nuclear power plants can come into contact with tritiated dust from stainless steel. This study aimed to investigate metal penetration and permeation after skin contamination with these particles. Static diffusion Franz cells were used with intact, damaged, or broken human skin.
View Article and Find Full Text PDFAnalysis of cellular effects by continuous exposure AT low concentration of tritium.
Radiat Prot Dosimetry
November 2024
Laboratory of Radiological Disasters and Medical Science, International Research Institute of Disaster Science, Tohoku University, 519-1176 Aramaki-aza-Aoba, Aoba-ku, Sendai 980-0845, Japan.
This study investigated the induction of DNA double-strand breaks (DSBs) in the hTERT-immortalized normal human diploid epithelial cells (RPE1-hTERT) continuously exposed to 6000 Bq/ml of tritiated water (HTO) and organically bound tritium (OBT). The relationship of the DSBs induction with the intracellular amount as well as the localization of tritium was also examined. Tritium-labeled thymidine (3H-Thy) and palmitic acid (3H-PA) were used as OBT.
View Article and Find Full Text PDFDevelopment of compact electrolytic enrichment system for environmental tritium analysis.
Radiat Prot Dosimetry
November 2024
Department of Nuclear and Radiochemical Science, Kyushu Environmental Evaluation Association, 1-10-1 Matsukadai, Higashiku Fukuoka 813-0004, Japan.
The new electrolytic enrichment system with compact glass cell was designed. Three (Ni-Fe-Ni) electrodes are used, and electrolysis is carried out at a rate of 2.45 g per h with constant current density of 120 mA per cm2.
View Article and Find Full Text PDFTritium transfer from seawater into marine organisms TFWT.
Radiat Prot Dosimetry
November 2024
Water Treatment Center Administration Group, Water Treatment Center, Fukushima Dai-ichi D&D Engineering Company, Tokyo Electric Power Company Holdings, Inc. 1-1-3 Uchisaiwai-cho, Chiyoda-ku, Tokyo 100-8560, Japan.
Tokyo Electric Power Company, TEPCO, has started tritiated water release into the Pacific Ocean. In order to reduce unreasonable rumor caused by tritium release, flounder, abalone, and sarggasum were exposed to tritium enriched seawater, and time dependent Tissues Free Water Tritium (TFWT) concentration was measured. Estimating the concentration of Organically Bound Tritium (OBT) is important to assess tritium impact because it has a longer biological half-life than TFWT.
View Article and Find Full Text PDFAssessing the variability of tissue-free water tritium and non-exchangeable organically bound tritium in pine needles in Fukushima using atmospheric titrated water vapor.
Sci Total Environ
October 2023
National Institute for Fusion Science, National Institutes of Natural Sciences, 322-6 Oroshi-cho, Toki-shi, Gifu 509-5292, Japan.
When conducting environmental tritium monitoring at nuclear and fusion facilities, it is important to understand how tritium concentrations vary within the soil-plant-atmosphere continuum. Past measurements of organically bound tritium (OBT) concentrations have been conducted from the standpoint of ascertaining the persistence of tritium in terrestrial vegetation, and it has been reported that OBT concentrations fluctuate depending on the influence of atmospheric sources and meteorological conditions. The present study provides information on the variability of tritium concentrations in vegetation growing close to atmospheric sources of tritiated water (HTO) in Japan.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!