AI Article Synopsis
- Phytophthora sojae is a major pathogen affecting soybeans, causing significant annual financial losses globally, prompting research into how it impacts soybean hypocotyls.
- Using proteomic techniques, 46 different proteins were found to be differentially expressed in the tolerant soybean line Yudou25 compared to the sensitive line NG6255, showcasing varying responses to infection.
- Findings highlight the importance of proteins related to energy regulation and disease defense, offering insights into plant responses during interactions with pathogens like P. sojae.
Article Abstract
Background: Phytophthora sojae causes soybean root and stem rot, resulting in an annual loss of 1-2 billion US dollars in soybean production worldwide. A proteomic technique was used to determine the effects on soybean hypocotyls of infection with P. sojae.
Results: In the present study, 46 differentially expressed proteins were identified in soybean hypocotyls infected with P. sojae, using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization tandem time of flight (MALDI-TOF/TOF). The expression levels of 26 proteins were significantly affected at various time points in the tolerant soybean line, Yudou25, (12 up-regulated and 14 down-regulated). In contrast, in the sensitive soybean line, NG6255, only 20 proteins were significantly affected (11 up-regulated and 9 down-regulated). Among these proteins, 26% were related to energy regulation, 15% to protein destination and storage, 11% to defense against disease, 11% to metabolism, 9% to protein synthesis, 4% to secondary metabolism, and 24% were of unknown function.
Conclusion: Our study provides important information on the use of proteomic methods for studying protein regulation during plant-oomycete interactions.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3180303 | PMC |
| http://dx.doi.org/10.1186/1477-5956-9-52 | DOI Listing |
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