One-step rapid immunochromatographic method for detection of diphtheria toxin in different water samples (phosphate buffer, milk, human nasopharyngeal swab) with the conjugate of monoclonal antibodies labeled with colloidal gold was developed. The limit of visible detection of the diphtheria toxin is 10 ng/ml and 15 min time analysis. The use of silver sensitivity enhancement and scanning equipment decreased the detection limit to 1.25 ng/ml.
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| http://dx.doi.org/10.1134/s1068162011030113 | DOI Listing |
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