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| http://dx.doi.org/10.1038/cr.2011.151 | DOI Listing |
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Inorganic arsenic modulates cell apoptosis by regulating Argonaute 2 expression via the p53 pathway.
Toxicol Res (Camb)
January 2025
Yunnan Provincial Key Laboratory of Public Health and Biosafety and School of Public Health, Kunming Medical University, No. 1168 Chunrongxi Road, Chenggong, Kunming, Yunnan 650500, China.
This study explores the role of Argonaute 2 (AGO2) in the induction of apoptosis by arsenic in 16HBE cells and investigates the association between AGO2 expression and arsenic exposure in a human population. By silencing AGO2 with siRNA, we examined its impact on cell viability and apoptosis using CCK-8, HO-PI, and JC-1 assays, complemented by qRT-PCR and Western blot analyses for gene and protein expressions. Our findings revealed a significant correlation between AGO2 expression and levels of exposure to inorganic arsenic (iAs), which was more pronounced than with other arsenic forms such as monomethylarsonic (MMA) and dimethylarsinic acids (DMA).
View Article and Find Full Text PDFInfectious myonecrosis virus (IMNV) induces upregulation of RNAi-related genes in white shrimp Penaeus vannamei.
Dev Comp Immunol
January 2025
Laboratório de Biologia Molecular, Instituto de Ciências Biológicas (ICB), Universidade Federal de Rio Grande (FURG), Av. Itália, Km 8, CEP 96203-900, Rio Grande, RS, Brazil.
Article Synopsis
- Infectious myonecrosis virus (IMNV) leads to considerable economic and social challenges in shrimp farming in the U.S. and Asia.
- This study focused on the transcriptional changes of key RNAi mechanism genes (Sid-1, Dicer-2, Argonaute-2) in Penaeus vannamei infected with varying concentrations of IMNV.
- Results showed that higher IMNV concentrations impacted transcription levels of these genes, but the overall viral load remained unchanged, suggesting Sid-1 mRNA expression as a potential marker for monitoring viral replication in shrimp farming.
Design, synthesis, molecular dynamics and gene silencing studies of novel therapeutic HIF-1α siRNAs in hypoxic cancer cells.
Int J Biol Macromol
December 2024
Department of Pharmaceutical Sciences, School of Pharmaceutical Sciences, Babasaheb Bhimrao Ambedkar University (A Central University), Vidya Vihar, Raebareli Road, Lucknow 226 025, Uttar Pradesh, India. Electronic address:
Hypoxia inducible factors (HIFs) are heterodimeric proteins that belong to a small group of transcription factors, which mainly regulates transcription of genes under hypoxic conditions. Particularly, oxygen sensing subunit of HIF-1α is a predominant subtype that heterodimerizes with oxygen-independent HIF-1β subunit, to trigger the transcription of hypoxia responsive genes. Due to poor supply of blood and rapid division of cancerous cells, tumor microenvironment exhibits low oxygen condition and therefore increased levels of HIF-1α.
View Article and Find Full Text PDFMEF2A is a transcription factor for circPVT1 and contributes to the malignancy of acute myeloid leukemia.
Int J Oncol
November 2024
Department of Hematology, Hematology Research Center of Yunnan Province, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan 650032, P.R. China.
Article Synopsis
- Acute myeloid leukemia (AML) has a high risk of relapse and poor survival, with key roles played by circPVT1 and MEF2A in cancer progression that are still being investigated.
- Research showed that circPVT1 is overexpressed in AML and is regulated by MEF2A, which enhances cell survival and promotes processes linked to cancer spread called epithelial-mesenchymal transition (EMT).
- CircPVT1 targets microRNA (miR)-455-3p, influencing the regulation of the MCL1 protein to facilitate cancer cell viability and EMT, suggesting that interrupting this pathway could be a potential therapeutic approach for AML.
Elucidating microRNA-34a organisation within human Argonaute-2 by dynamic nuclear polarisation-enhanced magic angle spinning NMR.
Nucleic Acids Res
October 2024
Department of Medical Biochemistry and Microbiology, Uppsala University, Husargatan 3, 75237 Uppsala, Sweden.
Understanding mRNA regulation by microRNA (miR) relies on the structural understanding of the RNA-induced silencing complex (RISC). Here, we elucidate the structural organisation of miR-34a, which is de-regulated in various cancers, in human Argonaute-2 (hAgo2), the effector protein in RISC. This analysis employs guanosine-specific isotopic labelling and dynamic nuclear polarisation (DNP)-enhanced Magic Angle Spinning (MAS) NMR.
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