In a multicenter study, we determined the expression profiles of 863 microRNAs by array analysis of 454 blood samples from human individuals with different cancers or noncancer diseases, and validated this 'miRNome' by quantitative real-time PCR. We detected consistently deregulated profiles for all tested diseases; pathway analysis confirmed disease association of the respective microRNAs. We observed significant correlations (P = 0.004) between the genomic location of disease-associated genetic variants and deregulated microRNAs.
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MicroRNA profiling from dried blood samples.
Crit Rev Clin Lab Sci
March 2019
a Institute of Human Genetics, Medical Faculty , Saarland University, Homburg , Germany.
MicroRNAs (miRNAs) hold great promise as blood-borne and circulating biomarkers for numerous diseases. However, the reliability of such liquid biopsies is particularly impacted by problems associated with the handling of biological liquids in the pre-analytical stage of biomarker processing. Dried blood spots (DBS) and other capillary blood microsampling devices offer a way to circumvent many of these complications.
View Article and Find Full Text PDFTechnical Stability and Biological Variability in MicroRNAs from Dried Blood Spots: A Lung Cancer Therapy-Monitoring Showcase.
Clin Chem
September 2017
Clinical Bioinformatics, Saarland University, Homburg, Germany;
Background: Different work flows have been proposed to use miRNAs as blood-borne biomarkers. In particular, the method used for collecting blood from patients can considerably influence the diagnostic results.
Methods: We explored whether dried blood spots (DBSs) facilitate stable miRNA measurements and compared its technical stability with biological variability.
Bias in High-Throughput Analysis of miRNAs and Implications for Biomarker Studies.
Anal Chem
February 2016
Chair for Clinical Bioinformatics, Saarland University, 66123 Saarbrücken, Germany.
A certain degree of bias in high-throughput molecular technologies including microarrays and next-generation sequencing (NGS) is known. To quantify the actual impact of the biomarker discovery platform on miRNA profiles, we first performed a meta-analysis: raw data of 1 539 microarrays and 705 NGS blood-borne miRNomes were statistically evaluated, suggesting a substantial influence of the technology on biomarker profiles. We observed highly significant dependency of the miRNA nucleotide composition on the expression level.
View Article and Find Full Text PDFWhat makes a blood cell based miRNA expression pattern disease specific?--a miRNome analysis of blood cell subsets in lung cancer patients and healthy controls.
Oncotarget
October 2014
Institute of Human Genetics, Medical School, Saarland University, Building 60, 66421 Homburg/Saar, Germany.
There is evidence of blood-borne miRNA signatures for various human diseases. To dissect the origin of disease-specific miRNA expression in human blood, we separately analyzed the miRNome of different immune cell subtypes, each in lung cancer patients and healthy individuals. Each immune cell type revealed a specific miRNA expression pattern also dependinging on the cell origin, line of defense, and function.
View Article and Find Full Text PDFToward the blood-borne miRNome of human diseases.
Nat Methods
September 2011
Biomarker Discovery Center, Heidelberg, Germany.
In a multicenter study, we determined the expression profiles of 863 microRNAs by array analysis of 454 blood samples from human individuals with different cancers or noncancer diseases, and validated this 'miRNome' by quantitative real-time PCR. We detected consistently deregulated profiles for all tested diseases; pathway analysis confirmed disease association of the respective microRNAs. We observed significant correlations (P = 0.
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