Two recombinant mutants of porcine circovirus type 2 (PCV2), which resulted from replacement of a genomic fragment containing the open reading frame 2 (ORF2) of genotype PCV2b with that of genotype PCV2a, were obtained initially from co-infection with PCV2a and 2b genotype viruses in vitro. The two mutant viruses contained the ORF1 sequence from genotype PCV2b and the ORF2 sequence from genotype PCV2a. They were designated according to the nomenclature proposed by Grau et al., indicating the origin of the ORF1 sequence first and that of the ORF2 sequence second, i.e., PCV2b(JF11)/2a(CL1) and PCV2b(YJ)/2a(CL1). The replication efficiencies of the two PCV2 recombinant mutants were enhanced significantly and their antigenicities were altered significantly in vitro when compared with their parental strains.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.virol.2011.08.004 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Newcastle Disease Virus Expressing Cap Gene of Porcine Circovirus Type 2 Confers Protection in Mice and Induced Long-Lasting Neutralizing Antibodies in Pigs.
Vaccines (Basel)
November 2024
Recombinant DNA Laboratory, Division of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, UP, India.
: Porcine Circovirus 2 (PCV2) infection poses significant health and economic challenges to the global swine industry. The disease in pigs leads to lymphoid depletion, resulting in immunosuppression and increased susceptibility to co-infections with other bacterial and viral pathogens. This study evaluated the efficacy of two novel recombinant Newcastle disease virus (NDV) strain R2B vectored vaccines that express the cap gene of PCV2 alone and along with the transmembrane and cytoplasmic tail (TMCT) domains of the NDV F gene.
View Article and Find Full Text PDFAn updated phylogeography and population dynamics of porcine circovirus 2 genotypes: are they reaching an equilibrium?
Front Microbiol
October 2024
Departament de Sanitat i Anatomia Animals, Facultat de Veterinària, UAB, Barcelona, Spain.
Introduction: Porcine circovirus 2 (PCV2) emerged more than three decades ago as one of the most impactful pathogens in the swine industry. Despite being a DNA virus, one of the hallmarks of PCV2 is its high evolutionary rate, which has led to the emergence of different genotypes, each exhibiting varying degrees of evolutionary success. Current knowledge suggests the occurrence of three main waves of genotype dominance, alternating over time (i.
View Article and Find Full Text PDFDetection of PCV2d in Vaccinated Pigs in Colombia and Prediction of Vaccine T Cell Epitope Coverage against Circulating Strains Using EpiCC Analysis.
Vaccines (Basel)
September 2024
Universidad Nacional de Colombia, Sede Bogotá, Facultad de Medicina Veterinaria y de Zootecnia, Centro de Investigación en Infectología e Inmunología Veterinaria (CI3V), Bogotá 111321, Colombia.
Article Synopsis
- Porcine circovirus type 2 (PCV2) is associated with porcine-circovirus-associated diseases (PCVADs), and while vaccination helps control these diseases, it does not provide complete immunity and may lead to vaccine failure.
- A study conducted in Colombia found that 82% of pigs and 92.9% of herds were infected with PCV2, with the PCV2d variant being the most prevalent.
- Histopathological examinations revealed common lung issues, and the study suggested that bivalent vaccines offer better immune coverage compared to monovalent vaccines despite ongoing clinical cases of PCV2.
Frequency and Genetic Analysis of Porcine Circovirus Type 2, Which Circulated between 2014 and 2021 in Jiangsu, China.
Animals (Basel)
October 2024
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Porcine circovirus-associated diseases, caused by porcine circovirus type 2 (PCV2), are widespread and result in significant economic losses to the global swine industry. PCV2 can currently be divided into nine genotypes (PCV2a to PCV2i), with the currently dominant one being the PCV2d genotype. In this study, 2675 samples from 804 pig farms in 13 cities in Jiangsu Province, China, were collected between 2014 and 2021 and subjected to polymerase chain reaction analysis to investigate the frequency and genetic diversity of PCV2.
View Article and Find Full Text PDFMolecular evolution and genotype shift of Porcine circoviruses type 2 in Vietnam.
Vet Res Commun
December 2024
Department of Infectious Disease and Veterinary Public Health, Faculty of Animal Husbandry and Veterinary Medicine, Nong Lam University, Ho Chi Minh City, Vietnam.
Porcine Circovirus Type 2 (PCV2), a significant pathogen in the global swine industry, causes Porcine Circovirus Associated Diseases (PCVAD), contributing to substantial economic losses. This study investigates the genetic diversity and evolutionary dynamics of PCV2 in Vietnam from 2007 to 2023. We sequenced and analyzed 47 PCV2 genomes isolated from swine farms across Vietnam between 2022 and 2023, revealing predominant circulation of PCV2d (80.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!