Real-time digital holographic microscopy of multiple and arbitrarily oriented planes.

Digital holographic microscopy is used to numerically refocus a recorded hologram at an arbitrary axial distance. However, as a straightforward property of coherent light fields, image reconstruction on an arbitrary tilted plane could be directly obtained by a rotation in k-space. We demonstrate that this property allows the real-time microscopic inspection of particle distribution over three mutually orthogonal planes at the same time. As a straightforward application we use the proposed technique for real-time monitoring of fluid flow over the three cross sections of a microfluidic channel.