AI Article Synopsis
- Good mitotic chromosome preparations are crucial for accurately studying chromosomal proteins through immunolocalization.
- While traditional fixation methods using methanol/acetic acid effectively maintain chromosome shape, they significantly decrease the amount of protein present.
- The authors propose new fixation and immunostaining techniques that enhance the quality of metaphase chromosomes and retain more chromosomal proteins, specifically from larval Drosophila brain cells.
Article Abstract
Good mitotic chromosome preparations are essential for the immunolocalization of chromosomal proteins. Although methanol/acetic acid fixation techniques preserve chromosome morphology very well, they remove a substantial fraction of chromosomal proteins. We have developed fixation/immunostaining procedures, described here, that are suitable for the immunolocalization of proteinaceous components of metaphase chromosomes from larval Drosophila brain cells. These procedures result in good chromosomal quality with minimal removal of proteins.
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| http://dx.doi.org/10.1101/pdb.prot065524 | DOI Listing |
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