AI Article Synopsis
- The Mph1 protein in budding yeast is similar to the human FANCM protein and has the ability to disrupt D-loop structures, which helps prevent chromosome crossovers during homologous recombination.
- Mph1 is involved in repairing DNA replication forks and can reverse these forks as well as process the Holliday junction through DNA branch migration.
- The protein also unwinds specific DNA structures related to the D-loop, highlighting its crucial role in fixing damaged replication forks and providing insights into its mechanisms.
Article Abstract
The budding yeast Mph1 protein, the putative ortholog of human FANCM, possesses a 3' to 5' DNA helicase activity and is capable of disrupting the D-loop structure to suppress chromosome arm crossovers in mitotic homologous recombination. Similar to FANCM, genetic studies have implicated Mph1 in DNA replication fork repair. Consistent with this genetic finding, we show here that Mph1 is able to mediate replication fork reversal, and to process the Holliday junction via DNA branch migration. Moreover, Mph1 unwinds 3' and 5' DNA Flap structures that bear key features of the D-loop. These biochemical results not only provide validation for a role of Mph1 in the repair of damaged replication forks, but they also offer mechanistic insights as to its ability to efficiently disrupt the D-loop intermediate.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3185188 | PMC |
| http://dx.doi.org/10.1016/j.dnarep.2011.08.002 | DOI Listing |
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