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Evaluation of the Efficacy of Disinfectants in Reducing Contamination of Bacterial and Viral Murine Pathogens on Gloved Hands.
J Am Assoc Lab Anim Sci
November 2024
1Research Animal Resources, University of Minnesota, Minneapolis, Minnesota.
Disinfectant application to gloved hands before handling SPF mice is standard practice to minimize transmission of pathogens and microbial contamination between cages. The risk of contamination with murine pathogens on gloves as well as the efficacy of disinfectant application for this step is largely unknown. This study aimed to determine if murine norovirus (MNV), Helicobacter spp.
View Article and Find Full Text PDFAssessing the prevalence of human enteric viruses in hospital wastewater to evaluate the effectiveness of wastewater treatment systems.
Ecotoxicol Environ Saf
December 2024
Department of Clinical Laboratory, No.906 Hospital of People's Liberation Army, Ningbo, China.
In this experiment, we employed Real-time PCR(RT-PCR) and metagenomic Next-Generation Sequencing (mNGS) techniques to detect the presence of Norovirus, Rotavirus Group A, Adenovirus Group F, and Astrovirus in untreated sewage from three major hospitals. A comparison with clinical lab test outcomes revealed Norovirus as having the highest infection rate, followed by Adenovirus Group F and Rotavirus Group A. Despite not testing for Astrovirus in clinical labs, its sewage detection rate was surpassed only by Norovirus, suggesting a potentially high clinical infection rate.
View Article and Find Full Text PDFComparing Two Seawater Temperatures For Human Norovirus Depuration From Oysters.
J Food Prot
November 2024
Ifremer, U. Microbiologie Aliment Santé Environnement, LSEM/RBE, Nantes, France.
Article Synopsis
- Shellfish-borne gastroenteritis outbreaks still happen even with regulations in place to monitor microbiological quality, primarily because human norovirus is not fully eliminated during oyster depuration.
- An experiment was conducted to see how seawater temperature affects the removal of norovirus from oysters, using temperatures of 8°C and 18°C with contaminated oysters.
- Results indicated that depuration at 8°C took longer (41 days) to reduce norovirus levels compared to 18°C (24 days), and also showed differences in how long various norovirus genogroups persisted in oysters.
Discovery of a new volcanic soil material, "Akahoya," as an adsorbent for bacterial and viral pathogens and its application to environmental purification.
Appl Environ Microbiol
September 2024
Center for Animal Disease Control, University of Miyazaki, Miyazaki, Japan.
Akahoya is a volcanic soil rich in alumina, primarily deposited in Kyushu, Japan. We have found that Akahoya adsorbs bacteria in the water surrounding cattle grazing areas, suggesting a potential for environmental purification. This study investigated the spectrum of microorganisms adsorbed by Akahoya using a column filled with Akahoya through which a suspension of microorganisms was passed.
View Article and Find Full Text PDFInactivation of murine norovirus and hepatitis A virus on various frozen fruits using pulsed light.
Int J Food Microbiol
November 2024
Institute of Nutrition and Functional Foods (INAF), Université Laval, Quebec City, QC, Canada. Electronic address:
The frozen fruit sector has experienced significant growth due to improved product quality as well as the advantage of long-term preservation. However, freezing alone does not eliminate foodborne viruses, a major public health concern and considerable economic burden. One promising disinfecting treatment is pulsed light, shown previously to inactivate hepatitis A virus (HAV) and murine norovirus-1 (MNV-1) on the surface of fresh berries.
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