Objective: To observe the effects of mesenteric lymph drainage and transfusion on pump activity and oxygen free radical of erythrocyte membrane of rats following hemorrhagic shock or normal, respectively.

Methods: Twenty-four male Wistar rats were randomly divided into sham-shock group, shock group, shock mesenteric lymph drainage group (drainage group) and shock mesenteric lymph transfusion group (transfusion group) by randomization, with 6 rats in each group. Model of hemorrhagic shock was reproduced both in shock group and drainage group after anesthesia and operation, mesenteric lymph was drained 1 hour after shock in drainage group. Sham-shock group only received anesthesia and laparotomy, and the obtained mesenteric lymph was transfused in transfusion group. Blood was obtained from abdominal aorta, and red cell membrane suspension was prepared after 3 hours of shock or corresponding time, then the indices of erythrocyte membrane pump activity and oxygen free radical were determined.

Results: Compared with sham-shock group, the activity of Na(+)-K(+)-ATPase (μmol×mg(-1)×h(-1)), Ca(2+)-ATPase (μmol×mg(-1) ×h(-1) ) and superoxide dismutase (SOD) (NU/mg)of red cell membrane were decreased markedly (Na(+)-K(+)-ATPase: 0.039±0.011 vs. 0.068±0.010; Ca(2+)-ATPase: 0.035±0.016 vs. 0.087±0.015; SOD: 0.785±0.289 vs. 1.202±0.328, P<0.05 or P<0.01), and the content of malondialdehyde (MDA, nmol/mg) was increased markedly in shock group (1.914±0.225 vs. 0.913±0.138, P<0.01). Compared with shock group, the activity of Na(+)-K(+)-ATPase (0.056±0.009), Ca(2+)-ATPase (0.079±0.025) and SOD (1.220±0.380) of red cell membrane were increased in drainage group, and the level of MDA (1.214±0.127) was decreased markedly (P<0.05 or P<0.01). Compared with sham-shock group, the activity of Na(+)-K(+)-ATPase (0.050±0.013), Ca(2+)-ATPase (0.056±0.023) of red cell membrane in transfusion group were significantly lower (both P<0.05), the content of MDA (1.456±0.270) was significantly higher (P<0.01), respectively, the activity of SOD (0.862±0.288) showed a lowering trend, but there was no statistical significance (P>0.05).

Conclusion: Mesenteric lymph return is a key factor in decreasing pump activity while aggravating free radical damage injury of erythrocyte membrane.

Download full-text PDF

Source

Publication Analysis

Top Keywords

mesenteric lymph
24
drainage group
16
group
13
pump activity
12
activity oxygen
12
oxygen free
12
free radical
12
erythrocyte membrane
12
sham-shock group
12
group shock
12

Similar Publications

Systemic sclerosis (SSc) is a complex autoimmune disease with an unclear etiology and no effective treatments. Recent research has suggested involvement of the microbiome in SSc pathogenesis. This study aimed to identify specific microbial species associated with SSc and explore their therapeutic potential.

View Article and Find Full Text PDF

can persist asymptomatically within tissues for extended periods. This remarkable feat is achieved through intricate host-pathogen interactions in immune cell aggregates called granulomas, wherein find favorable cellular niches to exploit while the host limits its expansion and tissue dissemination. Here, using a mouse model of persistent infection, we identify a host-protective role of eosinophils in control of Typhimurium ( Tm) infection within the mesenteric lymph nodes (MLN), the main lymphoid tissue of Tm persistence.

View Article and Find Full Text PDF

Aim: To assess transmural remission in patients with Crohn's disease using low-dose small bowel computed tomography (CT) perfusion scans.

Materials And Methods: Forty six patients were divided into active and remission phases based on Crohn's Disease Activity Index (CDAI) and C-reactive protein (CRP). Dual-source CT enterography with low-dose perfusion scans was conducted to generate perfusion parameter maps, including blood flow (BF), blood volume (BV), time to peak (TTP), mean transit time (MTT), and permeability of surface (PS).

View Article and Find Full Text PDF

BACKGROUND Obstructive jaundice is a common surgical issue caused by obstruction in the bile ducts, which can result from factors such as stones or cancers in the main bile duct. This study aimed to investigate the effects of carvacrol, a compound known for its strong antioxidant properties, on intestinal damage, liver damage, and bacterial translocation in an animal model of obstructive jaundice. METHODS The study utilized six groups of six Wistar Albino rats each.

View Article and Find Full Text PDF

The Gut-Lung Axis During Ethanol Exposure and a Bacterial Challenge.

Biomedicines

December 2024

Department of Inflammation and Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.

Susceptibility to and severity of pulmonary infections increase with ethanol consumption. We have previously shown that ethanol-induced changes in the gut microbiome disrupt gut homeostasis, allowing for the translocation of proinflammatory mediators into the circulation and eliciting an immune response in the lung. Additionally, targeting the gut with butyrate supplementation not only rescues ethanol-induced disruptions to gut health but also reverses aspects of immune dysregulation in the lungs.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!