Determination of the non protein amino acid β-N-methylamino-l-alanine in estuarine cyanobacteria by capillary electrophoresis.

Toxicon

LEGE, CIMAR/CIIMAR, Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, R. dos Bragas 289, 4050-123 Porto, Portugal.

Published: October 2011

A capillary electrophoretic method for the determination of the amino acid β-N-methylamino-l-alanine (BMAA) was achieved using a fused-silica capillary column (50 cm × 75 μm I.D.) filled with 5 mM sodium tetraborate solution (pH 9), with an applied voltage of 25 kV, at 25 °C. The method was then applied in quantifying BMAA in eighteen strains of lyophilized estuarine cyanobacteria, following amino acid extraction using 0.1 M trichloroacetic acid and 6 M hydrochloric acid, sequentially.

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http://dx.doi.org/10.1016/j.toxicon.2011.08.007DOI Listing

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