AI Article Synopsis
- CD46 is a complement regulatory protein important for reproduction, found on the inner acrosomal membrane of sperm from humans and some animals.
- Unlike other complement proteins, CD46 is not present on the plasma membrane of human sperm despite potential activating agents in the follicular fluid.
- Research using dual immunofluorescence suggests that in bovine sperm, CD46 may be located on the outer acrosomal membrane and possibly the plasma membrane, rather than the inner acrosomal membrane.
Article Abstract
Membrane cofactor protein (CD46) is complement regulatory protein with probable function in the reproduction process. Expression of CD46 on human, mice, rat and guinea pig spermatozoa is restricted to the inner acrosomal membrane. In spite of the presence of anti-sperm antibodies and other potential complement activating agents in follicular fluid, CD46 is not expressed on the plasma membrane of spermatozoa as the other complement regulatory proteins (DAF and CD59) in human. Using dual immunofluorescence labelling with mAb IVA-520 (anti-bovine CD46) and various lectins with different binding pattern or monoclonal antibody ACR.4, targeted against intra-acrosomal protein, we excluded the expression of CD46 on the inner acrosomal membrane as well as in the acrosomal content but, we suggested the localization of this molecule on the outer acrosomal membrane and possibly on the plasma membrane of bovine sperm.
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| http://dx.doi.org/10.4149/gpb_2011_SI1_70 | DOI Listing |
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