[Preparation of a hyaluronic acid modified bovine serum albumin nanoparticle and its anti-tumor effect].

Objective: To prepare hyaluronic acid modified bovine serum albumin nanoparticle, investigate the modification extent, the drug entrapment property and its anti-tumor effect.

Methods: Bovine serum albumin nanoparticles were prepared by desolvation method, then use hyaluronic acid to modify the surface of bovine serum albumin nanoparticle. The decrement of reactive amino groups on the surface of bovine serum albumin nanoparticles was applied to evaluate the modification extent of hyaluronic acid. Single factor was used to study the effects of several influencing factors in preparation on the modification extent of hyaluronic acid modified bovine serum albumin nanoparticles. The influence of pH on drug entrapment ratio as well as the drug entrapment property were studied. The inhibition rate of nanoparticle on HepG2 was evaluated with MTT asasay.

Results: The prepared hyaluronic acid modified bovine serum albumin nanoparticle had a average particle size of 396 nm, and Zeta potential of - 19.7 mV, the deceased ratio of surface reactive amino groups was 34.28%; hyaluronic acid modified mitoxantrone bovine serum albumin nanoparticles had a property of drug load ratio 11.13%, drug entrapment ratio 94.64% and 398 nm average particle size, Zeta potential - 17.9 mV, with a distinguished delayed drug release effect. A higher inhibition rate was observed for hyaluronic acid modified bovine serum albumin nanoparticle than that of mitoxantrone solution (P < 0.05).

Conclusion: The preparation technique is stable which could be used to prepare hyaluronic acid modified bovine serum albumin nanoparticle, and the prepared nanoparticle has a higer anti-tumor effect than that of mitoxantrone solution.