The field-effect enzymatic detection technique has been applied to the amperometric immunoassay of the cancer biomarker, carcinoma antigen 125 (CA 125). The detection adopted a reagentless approach, in which the analyte, CA 125, was immobilized on the detecting electrode, which was modified using carbon nanotubes, and the detection signal was obtained by measuring the reduction peak current of the enzyme that was used to label the antibody. A gating voltage was applied to the detecting electrode, inducing increase in the signal current and therefore providing amplification of the detection signal. The voltage-controlled signal amplification of the detection system has increased the sensitivity and lowered the detection limit of the system. A detection limit of 0.9U/ml was obtained in the work.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bios.2011.07.072 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A magneto-controlled microfluidic device for voltammetric immunoassay of carbohydrate antigen-125 with silver-polypyrrole nanotags.
Anal Methods
September 2020
College of Life Sciences, Fujian Normal University, Fuzhou 350117, Fujian, China.
An innovative magnetic immunoassay was developed for the voltammetric detection of carbohydrate antigen-125 (CA-125) on a home-made microfluidic device including a multisyringe pump, selection valve and magneto-controlled detection cell. Two kinds of biofunctionalized nanostructures including anti-CA-125 capture antibody-conjugated magnetic beads and anti-CA-125 detection antibody-labeled silver-polypyrrole (Ag-PPy) nanohybrids were utilized for a sandwiched immunoreaction in the presence of CA-125. With the help of an external magnet, the formed magnetic immunocomplexes were attached to the sensing interface to activate the electrical contact between Ag-PPy nanohybrids and the base electrode, thus resulting in the switching on of the sensor circuit for the generation of voltammetric signals thanks to electroactive Ag-PPy nanohybrids.
View Article and Find Full Text PDFImpact of carrier capacitance on Geobacter enrichment and direct interspecies electron transfer under anaerobic conditions.
Bioresour Technol
January 2025
State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, No. 73 Huanghe Road, Nangang District, Harbin 150090 China. Electronic address:
Direct interspecies electron transfer (DIET) enhances anaerobic digestion by facilitating electron exchange between electroactive bacteria and methanogenic archaea. While Geobacter species are recognized for donating electrons to methanogens via DIET, they are rarely detected in mixed microbial communities. This study examined various non-electrode biological carriers (zeolite, carbon cloth, activated carbon and biochar) to promote Geobacter cultivation under anaerobic conditions and identify pivotal factors influencing their symbiosis with methanogens.
View Article and Find Full Text PDFFabrication of TeNT/TeO heterojunction based sensor for ultrasensitive detection of NO.
J Hazard Mater
January 2025
School of Integrated Circuits, Dalian University of Technology, Dalian, Liaoning 116024, China. Electronic address:
Tellurium nanotubes (TeNT) heterojunction with Tellurium oxide (TeO) were prepared by in situ oxidation at elevated temperatures in air. The chemiresistive type NO sensor was then fabricated by depositing the synthesized TeNT/TeO on the integrated gold electrodes. The response of the TeNT/TeO based sensor to 600 ppb NO was 38.
View Article and Find Full Text PDFHarnessing Nanomaterials for Next-Generation DNA Methylation Biosensors.
Small
January 2025
Department of Clinical Laboratory, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, 310016, China.
DNA methylation is an epigenetic mechanism that regulates gene expression and is implicated in diseases such as cancer and atherosclerosis. However, traditional clinical methods for detecting DNA methylation often lack sensitivity and specificity, making early diagnosis challenging. Nanomaterials offer a solution with their unique properties, enabling highly sensitive photochemical and electrochemical detection techniques.
View Article and Find Full Text PDFAtomically Dispersed Ni-N-C Catalysts for Electrochemical CO Reduction.
Small
January 2025
Materials Physics and Applications Division, Los Alamos National Laboratory, Los Alamos, NM, 87545, USA.
The atomic dispersion of nickel in Ni-N-C catalysts is key for the selective generation of carbon monoxide through the electrochemical carbon dioxide reduction reaction (CORR). Herein, the study reports a highly selective, atomically dispersed Ni-N-C catalyst with reduced Ni loading compared to previous reports. Extensive materials characterization fails to detect Ni crystalline phases, reveals the highest concentration of atomically dispersed Ni metal, and confirms the presence of the proposed Ni-N active site at this reduced loading.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!