[Role of ERK in the hormesis induced by cadmium chloride in HEK293 cells].

Wei Sheng Yan Jiu

Department of Occupational Healthy, School of Public Health, Zhengzhou University, Zhengzhou 450001, China.

Published: July 2011

Objective: To detect the role of ERK in the hormesis induced by cadmium chloride in HEK293 cells.

Methods: HEK293 cells were treated with CdCl2 of 0, 0.0005, 0.005, 0.05, 0.5, 5, 50, 500 micromol/L for 12h and 24h. The ERK inhibitors (PD98059, U0126) and c-raf shRNA were added before the cell was treated with CdCl2. The proliferation viability was measured by thethiazolyl blue (MTT) and WST-8 assay. The level of the phosphorylated ERK and c-raf was examined with the western blot method. The level of c-fos, c-myc and c-raf mRNA was examined with RT-PCR.

Results: CdCl2 stimulated cell proliferation at lower concentrations (0.05 and 0.5 micromol/L) but inhibit it at higher concentrations (50 and 500 micromol/L). The c-fos mRNA increased significantly at low concentrations (0.51 micromol/L) and the c-myc mRNA increased with CdCl2 concentration. Treated with 100 micromol/L ERK1/2 inhibitor and c-raf shRNA, the cell proliferation did not increase significantly at low concentrations (0.05 and 0.51 micromol/L), but still decreased at high concentrations (50 and 500 micromol/L).

Conclusion: CdCl2 can induce the hormesis of HEK293 cell proliferation. The c-fos of ERK pathway may be involved in the biphasic effect induced by CdCl2.

Download full-text PDF

Source

Publication Analysis

Top Keywords

cell proliferation
12
erk hormesis
8
hormesis induced
8
induced cadmium
8
cadmium chloride
8
chloride hek293
8
treated cdcl2
8
500 micromol/l
8
c-raf shrna
8
shrna cell
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!