An acidic proteolytic enzyme, RVVX, was purified from Vipera russelli venom by successive chromatography on CM-Sephadex C-25, DEAE-cellulose and Sephadex G-100 columns. RVVX is a glycoprotein with a mol. wt of 79,000. It exhibited caseinolytic and factor X activating properties. Two trypsin inhibitors, TI-I and TI-II, were purified from V. russelli venom in a single step by CM-Sephadex C-25 column chromatography. The trypsin inhibitors interacted with the proteolytic enzyme RVVX. TI-I inhibited only the factor X activating property of RVVX while TI-II inhibited both, the caseinolytic and also factor X activating properties of RVVX. The edema inducing activity of RVVX increased markedly in the presence of non-edema inducing doses of TI-I and TI-II. RVVX, TI-I and TI-II were non-lethal in mice. The combination of RVVX and TI-II demonstrated enhanced toxicity.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0041-0101(90)90007-t | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Obscure properties of a traditional herb Pittosporum neelgherrense used to treat snakebite envenoming against Daboia russelli venoms.
Toxicon
November 2024
Department of Microbiology, Nehru Arts and Science College, Coimbatore, 641 005, Tamil Nadu, India.
In tropical nations, snakebite envenomation is a significant public health issue with negative human and social effects. This is due to three factors: 1) more species of the most hazardous snakes are present; 2) emergency medical assistance is not readily available; and 3) inadequate health care. The problems caused by snakebite envenomation have been partially resolved by immunotherapy.
View Article and Find Full Text PDFAscorbyl palmitate (ASC16) as a potential inhibitor of toxicity induced by Crotalus durissus terrificus venom.
Comp Biochem Physiol C Toxicol Pharmacol
October 2024
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Instituto de Química Básica y Aplicada del Nordeste Argentino (IQUIBA-NEA), W3404AAS Corrientes, Argentina; Facultad de Ciencias Exactas y Naturales y Agrimensura, Universidad Nacional del Nordeste, W3404AAS Corrientes, Argentina. Electronic address:
It is well known that C. d. terrificus venom causes pathophysiological effects such as neuropathies, coagulopathies, and even death.
View Article and Find Full Text PDFDaboialipase, a phospholipase A from Vipera russelli russelli venom posesses anti-platelet, anti-thrombin and anti-cancer properties.
Toxicon
February 2024
Department of Biological Sciences, BITS Pilani K K Birla Goa campus, Zuarinagar, 403726, India. Electronic address:
Snake venoms are known to contain toxins capable of interfering with normal physiological processes of victims. Specificity of toxins from snake venoms give scope to identify new molecules with therapeutic action and/or help to understand different cellular mechanisms. Russell's viper venom (RVV) is a mixture of many bioactive molecules with enzymatic and non-enzymatic proteins.
View Article and Find Full Text PDFSystematic review and meta-analysis on the efficacy of Indian polyvalent antivenom against the Indian snakes of clinical significance.
Arch Toxicol
February 2024
Biopharmaceutical Research Lab, Anusandhan Kendra-1, School of Chemical and Biotechnology, SASTRA Deemed-to-be-University, Thanjavur, Tamil Nadu, 613401, India.
Article Synopsis
- * The Indian polyvalent antivenom is designed to combat the venom from the most dangerous snakes—such as the Spectacled cobra and Russell's viper—but many other species also contribute to severe envenomations.
- * A systematic review and meta-analysis were performed to assess the neutralization efficiency of the antivenom against snake venoms, revealing significant variability in its effectiveness and highlighting factors affecting recognition of venom toxins.
Diosmin: A Daboia russelii venom PLAs inhibitor- purified, and characterized from Oxalis corniculata L medicinal plant.
J Ethnopharmacol
January 2024
Division of Biochemistry, School of Life Science, JSS Academy of Higher Education and Research, Mysore-15, India. Electronic address:
Ethnopharmacological Relevance: Oxalis corniculata L is a medicinal plant that belongs to the Oxalidaceae family. It is a little, slow-growing plant with a frail appearance typically found in mild temperate and tropical areas like Pakistan and India. This plant also includes many other bioactive substances, including alkaloids, flavonoids, terpenoids, cardiac glycosides, saponins, phlobatannins, and steroids.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!