UPLC-ion mobility spectrometry separations combined with mass spectrometry (UPLC-IM-MS) and tandem mass spectrometry (UPLC-IM-MS/MS) have been investigated for the simultaneous determination of testosterone and epitestosterone glucuronides in urine. The glucuronide epimers of testosterone and epitestosterone were separated by ion mobility spectrometry prior to mass analysis on the basis of differences in their collision cross sections, which have been measured in nitrogen. Combining ion mobility separation with UPLC/MS enhances the analysis of these low-abundance steroids in urine by selective interrogation of specific retention time, mass-to-charge and mobility regions. Detection limits for the UPLC-IM-MS/MS analysis of TG and ETG were 9.9 ng mL(-1) and 98 ng mL(-1) respectively, equivalent to 0.7 ng mL(-1) and 7.4 ng mL(-1) in urine, with linear dynamic ranges corresponding to 0.7-108 ng mL(-1) and 7.4-147 ng mL(-1) in urine. Repeatability (%RSD) for urine extracts was 0.64% and 2.31% for TG and ETG respectively.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1039/c1an15450h | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Detection of Oral Testosterone Undecanoate Administration in UGT2B117 del/del and del/ins Individuals. Part I: Urinary Steroid Profile and IRMS Markers.
Drug Test Anal
December 2024
Anti-Doping Laboratory, LSI Medience Corporation, Tokyo, Japan.
The detection of endogenous anabolic androgenic steroids misuse in Asian population using the Steroidal Module of the Athlete Biological Passport (ABP) is a challenge due to the high prevalence of UGT2B17 gene deletion polymorphism and low levels of testosterone (T) glucuronide. In this study, the capabilities of different approaches based on urine analysis for detecting oral T undecanoate administration were evaluated in 13 Asian volunteers, including 11 subjects with del/del genotype and 2 subjects with del/ins genotype. In this part of the work, the effect on the urinary steroid profile (SP) and the isotope ratio mass spectrometry (IRMS) markers were studied.
View Article and Find Full Text PDFMethylation of phase II metabolites of endogenous anabolic androgenic steroids to improve analytical performance.
Drug Test Anal
April 2024
Department of Pharmacy, UiT - The Arctic University of Norway, Tromsø, Norway.
The study of intact phase II metabolites of endogenous anabolic androgenic steroids (EAAS) gives important information about metabolism and has the potential to improve the detection of doping with testosterone. For analysis with liquid chromatography-mass spectrometry (LC-MS), chemical derivatization at the steroid moiety is a technique to improve the positive ionization efficiency of glucuronidated/sulfated EAAS under collision-induced dissociation (CID) conditions. However, regarding the chromatographic performance, there are still challenges to address, for example, poor peak shape, which is mainly caused by nondefined adsorption in the chromatographic system.
View Article and Find Full Text PDF5α-reduction of epitestosterone is catalysed by human SRD5A1 and SRD5A2 and increases androgen receptor transactivation.
J Steroid Biochem Mol Biol
July 2024
Institute of Metabolism and Systems Research, University of Birmingham, Birmingham B15 2TT, UK; MRC Laboratory of Medical Sciences, London W12 0HS, UK; Department of Biochemistry, Stellenbosch University, Stellenbosch 7600, South Africa.
Epitestosterone is a stereoisomer of the active androgen testosterone and its circulating concentrations are similar to those of testosterone in women and children. However, its biological function and pathways of metabolism remain unknown. The structural similarity to testosterone suggests a potential function in the modulation of androgen receptor signalling.
View Article and Find Full Text PDFEnhancing breast cancer screening with urinary biomarkers and Random Forest supervised classification: A comprehensive investigation.
J Pharm Biomed Anal
July 2024
Department of Chemistry, University of Turin, Italy; Centro Regionale Antidoping, Orbassano, TO, Italy. Electronic address:
Objectives: Urinary sex hormones are investigated as potential biomarkers for the early detection of breast cancer, aiming to evaluate their relevance and applicability, in combination with supervised machine-learning data analysis, toward the ultimate goal of extensive screening.
Methods: Sex hormones were determined on urine samples collected from 250 post-menopausal women (65 healthy - 185 with breast cancer, recruited among the clinical patients of Candiolo Cancer Institute FPO-IRCCS (Torino, Italy). Two analytical procedures based on UHPLC-MS/HRMS were developed and comprehensively validated to quantify 20 free and conjugated sex hormones from urine samples.
Biomarkers identification in follicular fluid in relation to live birth in in vitro fertilization of women with polycystic ovary syndrome in different subtypes by using UPLC-MS method.
Clin Chim Acta
April 2024
Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China; Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai 200011, China; Department of Obstetrics and Gynecology of Shanghai Medical School, Fudan University, Shanghai 200032, China. Electronic address:
Article Synopsis
- Polycystic ovary syndrome (PCOS) is a common infertility issue affecting women, but how it influences the metabolic profiles of follicular fluid (FF) in lean vs. obese women is not well understood.
- In a study involving 95 infertile women divided into four subgroups (lean and overweight controls and PCOS), researchers analyzed FF samples using advanced metabolomics techniques.
- The analysis identified 236 metabolites, revealing specific metabolic pathways that differ between PCOS and non-PCOS women, highlighting potential biomarkers that could predict live birth rates.*
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!