A simple and rapid isocratic, high performance liquid chromatography (HPLC) assay employing solid phase extraction (SPE) for the simultaneous determination of the anti HIV drug, efavirenz, the anti-tuberculosis drug, rifampicin and the desacetyl metabolite of rifampicin in plasma from HIV/tuberculosis infected patients has been developed. Using a Zorbax SB-Phenyl reverse-phase analytical column with UV detection, good separation and detection of the drugs was attained within a 10min run time. Intra- and inter-assay precision RSD values were found to be less than 15% at the concentrations examined (0.1-20μg/mL). The LOQ was found to be 0.1μg/mL for each agent and the assay was found to generate a linear response up to 20μg/mL. This low cost assay can accurately detect efavirenz and rifampicin concentrations within a clinically relevant concentration range using standard chromatography equipment, making it particularly applicable to resource-limited settings.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jpba.2011.07.041DOI Listing

Publication Analysis

Top Keywords

simultaneous determination
8
efavirenz rifampicin
8
rifampicin
5
determination efavirenz
4
rifampicin metabolite
4
metabolite desacetyl
4
desacetyl rifampicin
4
rifampicin levels
4
levels human
4
human plasma
4

Similar Publications

The molecular basis for the liquid-liquid phase separation (LLPS) behavior of various biomolecular components in the cell is the formation of multivalent and low-affinity interactions. When the content of these components exceeds a certain critical concentration, the molecules will spontaneously coalesce to form a new liquid phase; i.e.

View Article and Find Full Text PDF

Algae supplements are widely recognized for their nutritional benefits and are commonly marketed as natural health products. However, concerns regarding contamination with cyanobacterial toxins have been raised. Moreover, there is very little data regarding the potential contamination of algal supplements on the Chinese market by these toxins.

View Article and Find Full Text PDF

Unlabelled: Pediatric rapid response teams (PRRT) aim to detect the clinical deterioration of a patient and implement timely treatment, avoiding cardiopulmonary arrests (CPA) and in-hospital mortality.

Objective: To describe the experience with PRRT led by the pediatrician in a high-complexity hospital.

Patients And Methods: Descriptive, retrospective, longitudinal study.

View Article and Find Full Text PDF

An automated method was developed to simultaneously measure primary amines and short-chain aldehydes emission from foam and rubber samples in one experiment. The technique involved dynamic solid-phase microextraction (SPME) on-fiber derivatization coupled with a flow-cell unit. The parameters of the dynamic SPME on-fiber derivatization method were optimized, including SPME coating, derivatization agents loading temperature, loading time, and dynamic SPME extraction time.

View Article and Find Full Text PDF

Background And Objectives: Deep brain stimulation (DBS) is a well-established intervention for alleviating both motor and nonmotor symptoms of Parkinson disease. However, a common complication of stereotaxic DBS surgery is pneumocephalus, which can compromise electrode accuracy, complicate postoperative assessments, and negatively affect the long-term outcomes of DBS surgery. This report proposes a comprehensive and robust set of recommendations aimed at optimizing DBS surgical protocols to achieve zero pneumocephalus outcomes.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!