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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric behavior of succinoglycan monomers, dimers, and trimers isolated from Sinorhizobium meliloti 1021. | LitMetric

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric behavior of succinoglycan monomers, dimers, and trimers isolated from Sinorhizobium meliloti 1021.

Carbohydr Res

Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center & Center for Biotechnology Research in UBITA, Konkuk University, 1 Hwayang-dong Gwangjin-gu, Seoul 143-701, South Korea.

Published: October 2011

AI Article Synopsis

  • Researchers isolated low-molecular-weight succinoglycans (monomers, dimers, and trimers) from Sinorhizobium meliloti 1021 and studied them using MALDI-TOF MS with a specific matrix for analysis.
  • The primary components of these succinoglycans are formed from octasaccharide units, revealing interesting structural characteristics.
  • The analysis showed that galactosyl linkages are more unstable than glucosyl linkages, and that acetyl groups are more likely to break off than succinyl and pyruvyl linkages during the mass spectrometry process.

Article Abstract

Low-molecular-weight (LMW) succinoglycans (monomers, dimers, and trimers) were isolated from Sinorhizobium meliloti 1021 and have been firstly investigated by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) using 2,4,6-trihydroxyacetophenone (THAP) as an optimal matrix in the negative ion mode. The main fractions of LMW succinoglycans contain molecules assembled of octasaccharide subunits. MALDI-TOF mass spectra of the LMW succinoglycan monomers, the dimers, and the trimers showed the daughter ions resulting from the losses of the terminal galactose residues at the reducing ends, clearly indicating that the galactosyl linkages are more labile than the other glucosyl linkages. Furthermore, the losses of the acetyl groups as substituents rather than the succinyl and pyruvyl ester linkages by prompt fragmentation primarily occurred during MALDI-TOF analysis, suggesting the greater instability of acetyl linkages compared to pyruvyl and succinyl linkages.

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Source
http://dx.doi.org/10.1016/j.carres.2011.07.023DOI Listing

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