This paper describes a modified noninvasive microtest electrophysiological technology (NMT) for vacuolar H(+) flux detection. In this NMT system, the vacuole isolation procedure and buffer slope were modified, and the measuring errors from small spherical geometry were corrected. The trends in changes of vacuolar H(+) flux (ΔH(+) flux) after ATP or PP(i) supply calculated by NMT were consistent with the activities of V-ATPase and PPase measured by traditional methods. These findings indicate that our modified NMT is an appropriate method for vacuolar H(+) flux detection.
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| http://dx.doi.org/10.1016/j.ab.2011.07.018 | DOI Listing |
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