AI Article Synopsis
- - This study investigates how combining transforming growth factor-beta3 (TGF-β3) and hyaluronic acid (HA) affects the chondrogenic differentiation of human mesenchymal stem cells (hMSCs).
- - hMSCs were grown on different surfaces, and after four weeks, various cartilage-specific markers were analyzed to assess differentiation.
- - Results indicated that using HA-coated dishes along with TGF-β3 led to a significant increase in key cartilage markers, suggesting that both the cell-adhesion surface and the growth factor work together to enhance stem cell differentiation.
Article Abstract
This study was designed to evaluate the additive effects of transforming growth factor-beta3 (TGF-β3) and hyaluronic acid (HA) on chondrogenic differentiation of human mesenchymal stem cells (hMSCs). The hMSCs were cultured on collagen type I-, HA-, or fibronectin-coated cell culture dishes with or without TGF-β3 added to the culture medium. Four weeks after cell culture, chondrogenic differentiation of hMSCs was determined by evaluating the expression of cartilage-specific markers using real-time polymerase chain reaction, immunocytochemistry, and Western blot analysis. hMSCs cultured on HA-coated dishes with TGF-β3 supplementation revealed a prominent increase in collagen type II, aggrecan, and Sox9. When hMSCs were cultured without TGF-β3 supplementation, only hMSCs cultured on HA-coated dishes showed prominent expression of the cartilage-specific markers. This study shows that chondrogenic differentiation of hMSCs can be enhanced additively by interactions with both a specific cell-adhesion matrix and a soluble growth factor.
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