Evaluation of two magnetic-bead-based viral nucleic acid purification kits and three real-time reverse transcription-PCR reagent systems in two TaqMan assays for equine arteritis virus detection in semen.

Fabien Miszczak Kathleen M Shuck Zhengchun Lu Yun Young Go Jianqiang Zhang Stephen Sells Astrid Vabret Stéphane Pronost Guillaume Fortier Peter J Timoney Udeni B R Balasuriya

J Clin Microbiol

Frank Duncombe Laboratory, Animal Health Department, IFR146 ICORE, 14053 Caen, France.

Published: October 2011

This study showed that under specifically defined conditions with respect to nucleic acid extraction method and testing reagents, a previously described real-time reverse transcription-PCR (rRT-PCR) assay (T1 assay) provides sensitivity equal to or higher than that of virus isolation for the detection of equine arteritis virus in semen.

Download full-text PDF	Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3187312	PMC
http://dx.doi.org/10.1128/JCM.01187-11	DOI Listing

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