A 30 kDa antifungal protein was purified from red cabbage ( Brassica oleracea ) seeds. It exhibited a molecular mass and N-terminal amino acid sequence disinct from those of previously isolated Brassica antifungal proteins. The protocol used entailed ion exchange chromatography on Q-Sepharose and SP-Sepharose followed by fast protein liquid chromatography on Mono S. The protein hindered mycelial growth in Mycosphaerella arachidicola (with an IC50=5 μM), Setospaeria turcica, and Bipolaris maydis. It also inhibited the yeast Candida albicans with an IC50=96 μM. It exerted its antifungal action by permeabilizing the fungal membrane as evidenced by staining with Sytox green. The antifungal activity was stable from pH 3 to 11 and from 0 to 65 °C. It manifested antibacterial activity against Pseudomonas aeruginosa (IC50=53 μM). Furthermore, after 48 h of culture, it suppressed proliferation of nasopharyngeal cancer and hepatoma cells with IC50=50 and 90 μM, respectively.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/jf201874j | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The effect of supplemental LED lighting in the range of UV, blue, and red wavelengths at different ratios on the accumulation of phenolic compounds in pak choi and swiss chard.
Food Res Int
January 2025
Division Quality and Sensory of Plant Products, Georg-August-Universität Göttingen, Carl-Sprengel-Weg 1, 37075 Göttingen, Germany. Electronic address:
Phenolic compounds are known for their health-promoting effects on humans. Pak choi (Brassica rapa ssp. chinensis) and Swiss chard (Beta vulgaris subsp.
View Article and Find Full Text PDFCopigmentation effect on red cabbage anthocyanins, investigation of their cellular viability and interaction mechanism.
Food Res Int
January 2025
CSIR-Institute of Himalayan Bioresource Technology, Palampur, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India. Electronic address:
Anthocyanins (ANS) are an appealing substitute to synthetic colorants; but their practical applicability is limited due to low color stability. Copigmentation can improve both complex's color stability as well as intensity. In this study, we examined the interaction of red cabbage ANS with copigments i.
View Article and Find Full Text PDFHigh heterogeneity in the size distribution of the micellar fraction from in vitro digestions: sample preparation and reporting recommendations.
J Sci Food Agric
January 2025
Department of Food Technology, Fulda University of Applied Sciences, Fulda, Germany.
Background: Understanding the size and surface charge (ζ-potential) of particles in the mixed micellar fraction produced by in vitro digestion is crucial to understand their cellular absorption and transport. The inconsistent presentation of micellar size data, often limited to average particle diameter, makes comparison of studies difficult. The present study aimed to assess different size data representations (mean particle diameter, relative intensity- or volume-weighted size distribution) to better understand physiological mixed micelle characteristics and to provide recommendations for size reporting and sample handling.
View Article and Find Full Text PDFThe synergistic interaction effect between biochar and plant growth-promoting rhizobacteria on beneficial microbial communities in soil.
Front Plant Sci
December 2024
College of Plant Protection, Yunnan Agricultural University, Kunming, China.
Excessive use of chemical fertilizers and extensive farming can degrade soil properties so that leading to decline in crop yields. Combining plant growth-promoting rhizobacteria (PGPR) with biochar (BC) may be an alternative way to mitigate this situation. However, the proportion of PGPR and BC at which crop yield can be improved, as well as the improvement effect extent on different eco-geographic region and crops, remain unclear.
View Article and Find Full Text PDFEnhancement of yield and functional quality of Brassica microgreens: Effects of fertilization and substrate.
Food Chem
December 2024
Laboratorio de Cromatografía para Agroalimentos, Instituto de Biología Agrícola de Mendoza, CONICET- Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Almte. Brown 500, Chacras de Coria, Mendoza, Argentina; Cátedra de Química Analítica e Instrumental, Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Almte. Brown 500, Chacras de Coria, Mendoza, Argentina. Electronic address:
Brassica microgreens are rich in bioactive compounds, whose concentrations are influenced by environmental and cultivation conditions. This study evaluates the impact of different substrates and fertigation treatments, including sulfur, on the yield, morphology, and phytochemical profile of radish, red cabbage, white mustard, and red mizuna microgreens. Phytochemicals analyzed included total phenolic compounds (TPC), ascorbic acid (AA), and glucosinolates.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!