AI Article Synopsis
- In LC-MS/MS analysis, glucuronide conjugated metabolites can convert back to their parent drugs through a process called in-source collision-induced dissociation (CID).
- In the case of naproxen, it was found that its acylglucuronide undergoes significant CID, producing a strong signal for the parent naproxen drug ion, but this was not seen in other specific ion forms or in negative ESI conditions.
- The study suggests that bioanalytical scientists should monitor specific ion forms like [M-H](-) or [M+NH(4)](+) to reduce the effects of CID from acylglucuronides when analyzing these compounds.
Article Abstract
Background: In LC-MS/MS, glucuronide conjugated metabolites may convert back to the parent drug due to in-source collision-induced dissociation (CID).
Results: During the bioanalysis of naproxen, it was noticed that naproxen acylglucuronide exhibited intense in-source CID to the naproxen [M+H](+) ion under positive ESI. However, no in-source CID of the acylglucuronide to the naproxen [M+NH(4)](+) adduct was observed. Furthermore, absolutely no in-source CID was detected under negative ESI. This phenomenon was not only observed for naproxen acylglucuronide but for eight other acylglucuronides compounds.
Conclusion: We have shown that monitoring the parent drug [M-H](-) or [M+NH(4)](+) whenever possible could be an easy approach used by bioanalytical scientists to minimize the impact of in-source CID of acylglucuronides to the parent drug.
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| http://dx.doi.org/10.4155/bio.11.156 | DOI Listing |
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