Background: In Japan, a major type of home-related hypersensitivity pneumonitis (HP) is summer-type HP, which is caused by Trichosporon asahii (T. asahii) or Trichosporon mucoides. Some patients with home-related HP test negative for antibodies against Trichosporon; yet, a causative mold antigen cannot be identified.
Methods: We analyzed 19 patients with home-related HP, 8 healthy volunteers, and 35 patients with other diseases. We extracted DNA from cell pellets of bronchoalveolar lavage fluid (BALF), amplified the DNA by PCR using Trichosporon-specific primers or other fungus-specific primers, and cloned as well as sequenced the PCR amplicon. Other primers used were specific for Acremonium chrysogenum, Aspergillus fumigatus, Aspergillus niger, Fusarium napiforme, Humicola fuscoatra, Penicillium corylophilum, and Pezizia domiciliana.
Results: We detected Trichosporon DNA (n = 17) and F. napiforme DNA (n = 2) by PCR in 19 patients with home-related HP; however, these species were not identified in healthy volunteers. After sequencing of the PCR amplicon for Trichosporon species, we identified T. asahii (n = 11), Trichosporon japonicum (n = 1), and Cryptococcus uzbekistanesis (n = 4).
Conclusion: We could detect fungal DNA in BALF cell pellets from patients with home-related HP. These data suggest that this method might be useful to detect antigens responsible for home-related HP.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.rmed.2011.07.009 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Predictors of prolonged hospitalization among geriatric trauma patients using the modified 5-Item Frailty index in a Middle Eastern trauma center: an 11-year retrospective study.
Eur J Trauma Emerg Surg
January 2025
Department of Surgery, Trauma Surgery, Hamad Medical Corporation, Doha, Qatar.
Background: Using a validated tool, we explored the prevalence, risk factors, and predictors of longer hospitalization among hospitalized geriatric patients.
Methods: Retrospective and comparative analyses of age groups (55-64 vs. ≥ 65 years), gender, survival status, and frailty index categories were performed.
Treatment of Patients After Lung Transplantation With Covid Infection During Long-Term Follow-Up.
Transplant Proc
May 2024
Depatment of Cardiac Surgery, Medical University of Gdańsk, Poland.
Background: Patients undergoing lung transplantation are routinely managed with lifelong immunosuppression, which is associated with a heightened risk for infections. This study delves into the therapeutic challenges and strategies for managing lung transplant recipients (LTRs) infected with COVID-19 during long-term follow-up.
Methods: The was a case series analysis, among which nonstandard therapies consisting of targeted antibody treatment, antiviral drugs, or anti-interleukin-6 drugs were applied in patients after lung transplantation.
Prognostic Significance of Multifactorial Analysis in Complex Cervical Aero-Digestive Trauma Cases.
Medicina (Kaunas)
January 2024
Surgery Department, "Grigore T. Popa" University of Medicine and Pharmacy, 700115 Iasi, Romania.
: In the context of complex aerodigestive cervical traumas, the prognosis and outcome heavily depend on risk factors, particularly injuries to the larynx, trachea, major digestive tissues, cervical vertebrae, and vascular structures. With the increasing prevalence of trauma as a public health concern, there is a pressing need for epidemiological research and the implementation of preventative measures. The purpose of this research is to establish the profile of the predictable impact factors that determine the prognosis of patients with complex cervical trauma.
View Article and Find Full Text PDFSymptomatic Hypocalcemia due to Nutritional Vitamin D Deficiency in Three Adolescents during the COVID-19 Pandemic.
Case Rep Pediatr
October 2023
Division of Pediatric Endocrinology, Hasbro Children's Hospital, Providence, USA.
Background: Symptomatic hypocalcemia secondary to vitamin D deficiency (VDD) is rare among adolescents without underlying medical disorders, but its prevalence is higher in known risk populations. We report on three adolescent males with low nutritional intake of vitamin D and calcium and limited sun exposure who presented with hypocalcemic tetany and muscle cramps due to VDD during the COVID-19 pandemic. .
View Article and Find Full Text PDFFactors associated with home death in South Korea: Using the exit data from the Korean Longitudinal Study of Aging, 2008-2018.
PLoS One
July 2023
Department of Nursing Science, Sungshin Women's University, Seoul, Republic of Korea.
Background: Even though home deaths have been reported to improve quality of life, satisfy patients and families, and reduce healthcare expenditures, not enough is known about the factors that influence home deaths in Korea.
Objectives: This study aimed to examine the factors associated with home deaths among middle-aged and older adults in South Korea.
Methods: This secondary data analysis used core interview and exit interview data of the Korean Longitudinal Study of Aging conducted between 2008 and 2018.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!