AI Article Synopsis
- Glucokinase is an enzyme that converts glucose into glucose 6-phosphate by using ATP, and SgGlkA is a specific type found in the bacterium Streptomyces griseus that helps regulate carbon metabolism.
- Researchers expressed SgGlkA in E. coli, then purified and crystallized it using a specific method, achieving this at 293 K.
- They obtained a crystal of SgGlkA combined with glucose that diffracted X-rays at a high resolution of 1.84 Å, indicating a structured space group and specific unit-cell dimensions.
Article Abstract
Glucokinase catalyzes the phosphorylation of glucose using ATP to yield glucose 6-phosphate. SgGlkA is a bacterial group III glucokinase from Streptomyces griseus that seems to play a regulatory role in carbon catabolite repression in this organism. SgGlkA was expressed in Escherichia coli, purified and crystallized using the sitting-drop vapour-diffusion method at 293 K. A crystal of SgGlkA in complex with glucose was obtained using a reservoir solution consisting of 0.9 M sodium/potassium tartrate, 0.2 M NaCl and 0.1 M imidazole pH 8.1 and diffracted X-rays to 1.84 Å resolution. The crystal of SgGlkA in complex with glucose belonged to space group P6(2)22 or P6(4)22, with unit-cell parameters a = b = 109.19, c = 141.18 Å. The crystal contained one molecule in the asymmetric unit.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3151127 | PMC |
| http://dx.doi.org/10.1107/S1744309111022275 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Bridging the Gap Between Diabetes and Cardiovascular Disease: A Comparative Review of Different Glucagon-Like Peptide-1 (GLP-1) Agonists: Efficacy, Safety, and Patient Outcomes.
Cureus
November 2024
Family Medicine, King Abdulaziz Medical City Jeddah, Jeddah, SAU.
Diabetes mellitus, particularly Type 2 diabetes (T2DM) remains a significant concern globally with an increase in prevalence reported in recent years. If diabetes is not managed properly, it can lead to several complications including an increased risk of cardiovascular disease (CVD). Cardiovascular complications such as coronary heart disease, peripheral artery disease, and stroke are common among individuals with diabetes.
View Article and Find Full Text PDFBile acids as a key target: traditional Chinese medicine for precision management of insulin resistance in type 2 diabetes mellitus through the gut microbiota-bile acids axis.
Front Endocrinol (Lausanne)
December 2024
Shenzhen Hospital (Futian), Guangzhou University of Chinese Medicine, Shenzhen, China.
Type 2 diabetes mellitus (T2DM) is a chronic metabolic disease caused by insulin resistance (IR) and insufficient insulin secretion. Its characteristic pathophysiological processes involve the interaction of multiple mechanisms. In recent years, globally, the prevalence of T2DM has shown a sharp rise due to profound changes in socio-economic structure, the persistent influence of environmental factors, and the complex role of genetic background.
View Article and Find Full Text PDFThe impact of patient support and health education on diabetes management and glycemic control.
J Med Life
October 2024
Department of Family and Community Medicine, Prince Sultan Military Medical City (PSMMC), Riyadh, Saudi Arabia.
Diabetes mellitus is a chronic and complex medical condition that can lead to significant morbidity and mortality. Maintaining an adequate blood glucose level is important for patients with diabetes, and to improve glycemic control, patients need proper support and health education, which are essential components of comprehensive diabetes care. We used a rigorous approach based on the PRISMA and Cochrane Handbook principles, specifically focusing on randomized controlled trials (RCTs) published in English from 2005 onwards.
View Article and Find Full Text PDFData-driven discovery of the interplay between genetic and environmental factors in bacterial growth.
Commun Biol
December 2024
School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
A complex interplay of genetic and environmental factors influences bacterial growth. Understanding these interactions is crucial for insights into complex living systems. This study employs a data-driven approach to uncover the principles governing bacterial growth changes due to genetic and environmental variation.
View Article and Find Full Text PDFThermal Transfer Printed Flexible and Wearable Bionic Skin with Bilayer Nanofiber for Comfortable Multimodal Health Management.
Adv Healthc Mater
December 2024
Department of Biomedical Engineering, City University of Hong Kong, Kowloon, Hong Kong SAR, 999077, China.
The advent of bionic skin sensors represents a significant leap forward in the realm of wearable health monitoring technologies. Existing bionic skin technologies face several limitations, including complex and expensive manufacturing processes, low wearing comfort, and challenges in achieving comfortable real-time health monitoring. These shortcomings hinder the widespread adoption and practical utility of bionic skin in various applications.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!