Genomic and EST microsatellite markers for Aquilegia flabellata and cross-amplification in A. oxysepala (Ranunculaceae).

Premise Of The Study: The goal of this study was to identify genomic and expressed sequence tag (EST)-derived microsatellite markers from the species Aquilegia flabellata and assess their transferability in A. oxysepala.

Methods And Results: Eleven genomic and nine EST-derived microsatellite loci were characterized in A. flabellata. These microsatellite primers amplified 66 alleles in all 20 loci. The observed heterozygosity (H(O)) for each population ranged from 0.00 to 0.94.

Conclusions: All of the 20 loci were successfully amplified in A. oxysepala. These genomic and EST-derived microsatellite markers will be useful in further genetic structure, speciation, and adaptive evolution studies.