The monitoring of NVs in municipal wastewater by both real-time RT-LAMP and real-time RT-PCR, and the comparison of these two methods with respect to NV detection were carried out. The change in NVs detected by real-time RT-LAMP agreed well with that detected by real-time RT-PCR. In contrast, the correlation between the copy number determined by real-time RT-PCR and the threshold time (Tt) determined by real-time RT-LAMP obtained during monitoring was not significant (0.1
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http://dx.doi.org/10.1016/j.jbiosc.2011.06.012 | DOI Listing |
Biotechnol J
January 2025
School of Biology and Biological Engineering, South China University of Technology, Guangzhou, China.
Loop-mediated isothermal amplification (LAMP) is a detection method widely used in pathogen detection and clinical diagnosis. Nevertheless, it is highly constrained by thermal stability, catalytic activity, and resistance to inhibitors of Bst DNA polymerase. In this study, a novel DNA polymerase was characterized from Clostridium thermocellum, exhibiting potential in LAMP detection.
View Article and Find Full Text PDFFront Plant Sci
December 2024
College of Life Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.
Introduction: f. sp. (Fol) is one of the most devastating plant pathogenic fungi, the causal agent of root rot for tractylides macrocephala Koidz (AMK).
View Article and Find Full Text PDFAppl Microbiol Biotechnol
December 2024
Laboratório de Pesquisa em Malária, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil.
Animals (Basel)
November 2024
College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Duck Tembusu virus (DTMUV) is an emerging flavivirus that has inflicted significant economic losses on China's poultry industry. Rapid and accurate detection of DTMUV is crucial for effective prevention and control measures. In this study, we developed a novel, rapid visual detection assay that combines reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) with the CRISPR/Cas12a system for on-site detection of DTMUV.
View Article and Find Full Text PDFJ Food Sci
December 2024
Key Lab of Agricultural Products Low Carbon Cold Chain (Co-construction of ministry and province), Ministry of Agriculture and Rural Affairs, Tianjin University of Commerce, Tianjin, China.
Clostridium perfringens is a significant foodborne pathogen in chicken products. Rapid on-site detection of C. perfringens is crucial for mitigating the incidence of foodborne illnesses by enabling the prompt identification and recall of contaminated food products.
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