AI Article Synopsis
- Antimicrobial peptides (AMP) are crucial for protecting epithelial surfaces from harmful microorganisms, with significant differences noted in their expression between oral and extraoral tissues.
- Research utilizing real-time PCR, immunohistochemistry, and western blot techniques demonstrated that hBD-1, hBD-2, hBD-3, and psoriasin are expressed more in the oral mucosa than in skin, suggesting a stronger protective role in the mouth.
- Findings indicate potential for developing new treatments based on AMP derivatives, particularly targeting the variances in their expression levels between these two types of epithelium.
Article Abstract
Antimicrobial peptides (AMP) defend epithelial surfaces against pathological micro-organisms. We know of no comparison of their expression between the oral mucosa and extraoral epithelium, but knowledge of differences in their quantities is of interest, possibly as a starting point for new treatments. Expression of AMP human beta-defensin (hBD)-1/-2/-3 and psoriasin in the oral mucosa and extraoral epithelium of the head and neck were measured by real-time polymerase chain reaction (RT-PCR) (n=14), immunohistochemistry (n=6), and western blot (n=8). RT-PCR showed that all the genes investigated were expressed significantly more in the oral mucosa than in the skin (hBD-1: p=0.002; hBD-2: p=0.006; hBD-3: p=0.035; psoriasin: p=0.02). Immunohistochemistry and western blot showed differential concentrations of proteins: hBD-2 (p=0.021) and hBD-3 (p=0.043) were pronounced in the oral mucosa, whereas psoriasin was raised in the extraoral skin (p=0.021). There was no difference in protein concentrations for hBD-1 (p=0.08). The observed differences in the expression of AMP may be important for new treatments such as topical application of AMP derivatives.
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| http://dx.doi.org/10.1016/j.bjoms.2011.07.006 | DOI Listing |
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